二十八烷醇对小鼠耐缺氧作用的实验研究

目的:观察二十八烷醇对小鼠急性缺氧存活时间的影响。方法:雄性昆明种小鼠随机分为二十八烷醇组和对照组:二十八烷醇组小鼠给予不同剂量二十八烷醇(10mg.kg-1、50mg.kg-1、100mg.kg-1)灌胃,对照组小鼠给予相同体积的食用调和油灌胃,连续灌胃7d。观察两组小鼠密闭缺氧耐受时间、亚硝酸钠中毒存活时间、断头小鼠张口动作持续时间、注射异丙肾上腺素后密闭缺氧耐受时间,评价二十八烷醇对小鼠缺氧耐受性的影响。结果:给予10mg.kg-1和50mg.kg-1二十八烷醇组小鼠密闭缺氧耐受时间显著高于对照组(P<0.01),给予50mg.kg-1二十八烷醇组小鼠断头处死张口动作持续时间、注射异丙肾上腺素密闭缺氧耐受时间显著高于对照组(P<0.05),两组小鼠亚硝酸钠中毒存活时间没有显著差异(P>0.05)。结论:二十八烷醇可显著提高小鼠对于常压密闭缺氧、大脑缺血缺氧和心肌缺氧的耐受能力。

Experimental study on effects of l-octacosanol on hypoxic tolerance in mice

Objective:To evaluate the antihypoxic effects of l-octacosanol in mice.Methods:Male Kunming mice were randomly divided into l-octacosanol group and control group. Doses of 100 mg/kg, 50 mg/kg and 10 mg/kg l-octacosanol were administered to the l-octacosanol group and salad oil of the same volume was administered to the control group for 7 days. Then the tolerance time to isobaric hypoxia, survial time after sodium nitrite poisoning, persistence time of gape action after decapitation and tolerance time to isobaric hypoxia after administration of isoprinosine in both groups were recorded, respectively.Results:The tolerance time to isobaric hypoxia(P<0.01), persistence time of gape action after decapitation(P<0.05) and tolerance time to isobaric hypoxia after administration of isoprinosine(P<0.05) in l-octacosanol group were significantly longer than those in the control group. No significant difference of survial time after sodium nitric poisoning was found between two groups.Conclusion:l-Octacosanol can promote tolerance of mice to isobaric hypoxia, cerebral ischemic hypoxia and myocardial hypoxia.