| MSP扩增中3种DNA提取法的比较 |
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| 引用本文： | 张云,陈凯锋,刘彬,张晓兵.MSP扩增中3种DNA提取法的比较[J].西南国防医药,2010,20(1):27-28. |
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| 作者姓名： | 张云 陈凯锋 刘彬 张晓兵 |
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| 作者单位： | 乐山,解放军42医院检验科,四川,614100 |
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| 摘 要： | 目的比较标准酚-氯仿、TripureDNA提取法、强碱法3种DNA提取法，研究哪种方法更简单方便，更适合做DNA甲基化特异性PCR（MSP）。方法分别用标准酚-氯仿、TripureDNA提取法、强碱法提取Jurkat细胞株中的DNA，并对3种方法提取的DNA含量、纯度及所需时间进行比较。结果3种方法提取DNA的纯度和完整性都较好，标准酚-氯仿法提取的DNA含量最低，且最费时；强碱法提取的DNA含量最高，并最省时。结论这3种DNA提取法提取的DNA都能够达到做MSP的要求，比较而言，强碱法更具优越性。
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| 关 键 词： | DNA提取 DNA甲基化 Jurkat细胞株 |
Comparison of three DNA extraction methods for MSP amplification |
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| Zhang Yun,Chen Kaifeng,Liu Bin,Zhang Xiaobing.Comparison of three DNA extraction methods for MSP amplification[J].Medical Journal of National Defending forces in Southwest China,2010,20(1):27-28. |
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| Authors: | Zhang Yun Chen Kaifeng Liu Bin Zhang Xiaobing |
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| Institution: | Zhang Yun,Chen Kaifeng,Liu Bin,Zhang Xiaobing Department of Laboratory,Hospital 42 of PLA,Leshan,Sichuan,614100,China |
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| Abstract: | Objective To compare the standard phenol-chloroform method,Tripure DNA extraction method and alkali method in order to examine which one is more simple,convenient and suitable for DNA methylation-specific PCR(MSP).Methods DNA in Jurkat cell line was extracted by standard phenol-chloroform,Tripure DNA extraction method and alkali extraction method,respectively.Then the content and purity of DNA as well as extractive time were compared.Results The purity and integrity of DNA extracted by these three methods w... |
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| Keywords: | DNA extraction DNA methylation Jurkat cell line |
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