| 长效重组人超氧化物歧化酶的研制及其辐射防护作用 |
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| 引用本文： | 骆训懿,王晶翼,周丽君,陈宇萍,陈晓穗,朱迎春,刘晓琳,谢邦铁,李战青.长效重组人超氧化物歧化酶的研制及其辐射防护作用[J].海军总医院学报,1994(2). |
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| 作者姓名： | 骆训懿 王晶翼 周丽君 陈宇萍 陈晓穗 朱迎春 刘晓琳 谢邦铁 李战青 |
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| 作者单位： | 海军总医院分子生物学研究室 |
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| 摘 要： | 选用无毒、无免疫原性的高分子化合物聚乙二醇(PEG)为修饰剂,以活性酯法制备出PEG- 重组人超氧化物歧化酶(PEG-rh SOD)共价结合物.修饰酶的生物半衰期为15h,比修饰前延长了90倍,对酸、碱、热的耐受力明显高于修饰前.用照射小鼠的活存率评价了PEG-rhSOD的辐射防护作用.小鼠受8.5Gy γ射线照射前1h腹腔注射30万U/kg PEG-rh SOD,可提高活存率42%,与未修饰的rhSOD相比,活存率提高14%.实验仍在进行中.
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| 关 键 词： | 辐射防护剂 超氧化物歧化酶 基因工程 化学修饰 |
Preparation of long half-life recombinent human superoxid dismutase and a study its radioprotec-tive effect. |
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| Luo Xunyi et al.Preparation of long half-life recombinent human superoxid dismutase and a study its radioprotec-tive effect.[J].Journal of Naval General Hospital of PLA,1994(2). |
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| Authors: | Luo Xunyi |
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| Institution: | Luo Xunyi et al |
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| Abstract: | Recombinent human CuZn SOD (rhCu/Zn SOD) was covalently linked with activated polyethyleneglycol (PEG) ,a kind of nontoxic ,nonimmunogenic and biocompatible polymer. The modified enzyme,PEG-rhCu/Zn SOD,Has longer half-life (15 h). Its abilities to resist acid, alkali and heat were increasedsignificantly. Mice given PEG-rhCu/Zn SOD 1 h before irradiation had higher survival rate than thosenot given or given rhCu/Zn SOD after they were exposed to whole body r-ray irradiation at doses of 8.5Gy. |
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| Keywords: | Radioprotective agent Superoxide dismutase Genetic engineerihg Chemical modification |
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