| 人巨细胞病毒潜伏感染状态下编码miRNA的研究 |
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| 引用本文： | 宋鑫,张艳宇,邓江,肖军,吕丽萍,高博,马平,毕静媛,周锡鹏,许金波.人巨细胞病毒潜伏感染状态下编码miRNA的研究[J].军事医学,2016(4):311-315. |
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| 作者姓名： | 宋鑫 张艳宇 邓江 肖军 吕丽萍 高博 马平 毕静媛 周锡鹏 许金波 |
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| 作者单位： | 军事医学科学院野战输血研究所血液安全保障技术研究北京市重点实验室,北京,100850 |
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| 基金项目： | 北京市重点实验室科学技术基金资助项目(Z141102004414034) |
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| 摘 要： | 目的：探索潜伏感染状态下人巨细胞病毒（HCMV）所编码微小RNA（microRNA，miRNA），为研究其在HCMV病毒学和潜伏感染机制奠定基础。方法通过HCMV感染THP－1细胞构建HCMV潜伏感染细胞模型，采用高通量Solexa测序平台对其深度测序，并利用 RNAFold 等生物信息学软件进行二级结构预测和命名。结果HCMV在潜伏感染状态表达了miR－US25－2－3p、miR－US25－2－5p、miR－UL112、miR－US25－1、miR－UL22A 以及长度为25 bp的编号为PC－5p－148467的miRNA，对PC－5p－148467进行系列分析，将其命名为hcmv－miR－US33as－5p。结论在潜伏感染状态下，HCMV亦可编码多种miRNA。
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| 关 键 词： | 人巨细胞病毒 潜伏感染 深度测序 生物信息学 微小RNA |
HCMV-encoded miRNA during latent infection |
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| Authors: | SONG Xin;ZHANG Yan-yu;DENG Jiang;XIAO Jun;L Li-ping;GAO Bo;MA Ping;BI Jing-yuan;ZHOU Xi-peng;XU Jin-bo |
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| Institution: | SONG Xin;ZHANG Yan-yu;DENG Jiang;XIAO Jun;L Li-ping;GAO Bo;MA Ping;BI Jing-yuan;ZHOU Xi-peng;XU Jin-bo;Beijing Key Laboratory of Blood Safety and Supply Technologies,Institute of Transfusion Medicine,Academy of Military Medical Sciences; |
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| Abstract: | Objective To explore humam cytomegalovirus(HCMV) encoded microRNAs during latent infection in order to help study HCMV virology and latent infection mechanisms.Methods A model of HCMV latent infection via THP-1 cells infected with HCMV was constructed.Deep-sequencing was performed using high-resolution Solexa sequencing platform.The secondary structure of the newly sequenced miRNA was predicted by RNAFold bioinformatics software. Results HCMV encoded various miRNAs during latent infection, including miR-US25-2-3p, miR-US25-2-5p, miR-UL112, miR-US25-1, miR-UL22A and PC-5p-148467 with a predicted length of 25 bp, named hcmv-miR-US33as-5p.Conclusion HCMV can express many types of miRNAs during latent infection. |
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| Keywords: | human cytomegalovirus latent infection deep-sequencing bioinformatics microRNA |
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