腹部开放伤后海水浸泡对实验犬肠屏障功能的影响

目的 探讨腹部开放伤后海水浸泡对实验犬肠屏障功能的影响.方法 实验动物致伤后随机分为对照组(n=10)和海水浸泡组(n=10).对照组致伤后直接观察,海水浸泡组于致伤后立即放入人工配制的海水中,于致伤前(0 h)及出水后4、8、12、24 h取血测定血浆内毒素、谷氨酰胺、D-乳酸、双胺氧化酶(DAO)含量.实验后取门静脉血、肠系膜淋巴结进行细菌培养,并取小肠组织进行病理检查.致伤后给予乳果糖/甘露醇(L/M)肠内注入,收集24 h尿测定L/M含量.结果 海水浸泡组血清D-乳酸、DAO活性呈进行性上升趋势,8 h后与对照组比较差异有统计学意义(P＜0.05);谷氨酰胺呈进行性下降,12 h后与对照组比较差异有统计学意义(P＜0.01);内毒素升高,12 h后与对照组比较差异有统计学意义(P＜0.05);24 h后尿L/M与对照组比较差异有统计学意义(P＜0.05);门静脉和肠系膜淋巴结细菌培养,海水浸泡组出现大量菌落,与对照组比较差异有统计学意义(P＜0.01).病理结果显示,海水浸泡组与对照组比较肠黏膜损伤明显,黏膜厚度和绒毛高度均显著减小.结论 海水浸泡可导致实验犬的肠道屏障功能受损.

Effect of seawater immersion on intestinal barrier following open abdominal injury in dogs

Objective To explore the effect of seawater immersion on intestinal barrier following open abdominal injury in dogs. Methods Twenty adult mongrel dogs were randomly divided into the control group (n = 10) and the seawater immersion group (n = 10). The control group was immediately observed under microscope following abdominal injury, while the seawater immersion group was instantly put into the manually prepared seawater after abdominal injury. Blood samples were taken before injury (0 hour) and at 4, 8, 12,and 24 hours after injury for detection of endotoxin, glutamine, D-lactate, diamine oxidase (DAO). Samples of 24-hour urine were collected for measurement of lactulose / mannitol (L/M) ratio. The intestinal tissue samples were obtained 24 hours after seawater immersion for pathological examination. Results Compared with the control group, the seawater immersion group showed clear signs of intestinal barrier dysfunction. Serum Dlactate, and DAO activity tended to increase progressively, and significant differences could be noted 8 hours after immersion ( P ＜ 0. 05 ). However, glutamine was detected to be declining, and significant differences could be observed at hour 12 following seawater immersion, when compared with that of the control group (P ＜0. 01 ). Plasma endotoxin level of the seawater immersion group increased significantly 12 hours after seawater immersion when compared with that of the control group (P ＜ 0. 05 ). The 24-hour urine L/M ratio also showed significant differences ( P ＜ 0. 05 ). For the seawater immersion group, portal vein and mesenteric lymph node hacterial culture showed that there was a substantial colony, and significant differences could be seen, when a comparison was made between the two groups (P ＜0.01 ). Pathological examination indicated that the seawater immersion group had significant lesion in intestinal mueosa, as evidenced by a significant reduction of mucosal thickness and villous height ( P ＜ 0. 05 ), when a comparison was made between the 2 groups. Conclusions Seawater immersion could induce lesion to the intestinal barrier function following open abdominal injury in dogs.