高压氧对家兔随意皮瓣血管内皮生长因子和缝隙连接蛋白的影响

目的 探讨高压氧对家兔随意皮瓣毛细血管增殖作用的机制.方法 建立家兔背部随意皮瓣模型,予高压氧治疗,观察第3天和第7天时对照组和高压氧组皮瓣中毛细血管密度及血管内皮细胞生长因子(VEGF)、缝隙连接蛋白(CX43)的含量变化情况.结果 高乐氧组皮瓣毛细血管密度(第3天11.63±2.94,第7天13.84±3.49)明显高于对照组(第3天9.13±2.00.第7天10.51±3.01)(第3天时P<0.05,第7天时P<0.01);第3天和第7天时高压氧组皮瓣VEGF(第3天5.50±1.68,第7天5.81±1.64)表达明显高于对照组(第3天4.55±1.06,第7天4.63±1.24)(分别为P<0.01);高压氧组皮瓣CX43(第3天4.08±0.88,第7天4.60±0.97)表达明显高于对照组(第3天3.28±0.68,第7天3.60±1.01)(第3天时P<0.01,第7天时P<0.05).结论 高压氧治疗促进家兔随意皮瓣毛细血管增殖的作用机制之一可能是通过增加皮瓣组织中VEGF、CX43的表达而促进新生毛细血管形成.

Effects of hyperbaric oxygen on vascular endothelial growth factor and connexin 43 in random skin flaps of rabbits

Objective To explore effects and mechanism of hyperbaric oxygen (HBO) on capillary proliferation of random skin flaps in rabbits. Methods Random skin flap models were developed on the back of rabbits and HBO was administered to rabbits in the HBO group. Capillary density and contents of VEGF, CX43 were observed on the 3rd day and the 7th day respectively. Results Capillary density of the animals in the HBO group were higher than those in the control group on the 3rd day (P<0.05) and the 7th day (P<0.01) respectively. The content of VEGF in the HBO group was higher than that in the control group on the 3rd day and the 7th day (P<0.05). The contents of CX43 in the HBO group were higher than that in the control group on 3rd day (P<0.01) and the 7th day (P<0.05) respectively. Conclusions One of the mechanisms of HBO might be that higher expression of VEGF and CX43 promoted capillary proliferation in random skin flaps.