中药八味抗纤方抗大鼠肝纤维化的实验研究

 目的 观察中药八味抗纤方对四氯化碳诱导的肝纤维化大鼠的治疗作用.方法 采用四氯化碳橄榄油溶液腹腔注射制作大鼠肝纤维化模型,同时予不同剂量中药八味抗纤方灌胃,观察八味抗纤方对慢性肝损伤大鼠肝功能的影响, 采用Ishak肝病组织病变计分修正方案,结合HE染色、Masson染色和免疫组化染色研究八味抗纤方对大鼠肝组织病理改变的影响,以活化HSC特异性标记α-SMA标记显示活化肝星状细胞(hepatic stellate cell,HSC),探讨八味抗纤方抗肝纤维化的可能的作用机制.结果 低剂量组和高剂量组大鼠血清AST均显著低于模型组;肝纤维化程度轻于模型组;α-SMA阳性细胞数少于模型组.结论 八味抗纤方能够抑制四氯化碳诱导的大鼠肝纤维化,减轻纤维结缔组织增生,抑制HSC的活化.

An experimental study on Eight-Ingredient Decoction of Anti-Fibrosis in treatment of liver fibrosis

Objectives To study the therapeutic effect of Eight - Ingredient Decoction of Anti - Fibrosis on experimental liver fibrosis induced by carbon tetrachloride （CCl4 ）. Methods Carbon tetrachloride mixed with olive oil was injected intraperitoneally for 16 times to develop the rat model of liver fibrosis. Since the beginning of the model establishment, the rats were administered with a high dose of TCM and a low dose of TCM separately. The effect of decoction on ALT and AST in the rats with hepatic injuries was observed. According to Ishak liver disease pathological changes correction scoring scheme, HE stain, Masson stain and immunohistochemistry stain were used to study the effect of the Eight - Ingredient Decoction of Anti - Fibrosis on rats＇liver fibrosis, α - SMA immunohistochemistry stain was used to mark the activating HSC. The probable mechanism of the Eight - Ingredient Decoction of Anti - Fibrosis against liver fibrosis was discussed. Results The AST level in both groups with a high TCM dose and low TCM dose was significantly lower than that in the model control group. Both levels of inflammation and fibrosis in the groups with a high TCM dose and low TCM dose were significantly lower than those of the model control group. The number of α- SMA positive cells was smaller in the TCM groups than in the model control group. Conclusions The Eight - Ingredient Decoction of Anti - Fibrosis inhibits rat liver fibrosis induced by CCl4 , fiber connective tissue hyperplasia and formation of pseudo - lobe. The mechanism is likely to be the inhibition of hepatic stellate cell activation and collagen synthesis to protect hepatic ceils.