阿克替苷的体内外抗肿瘤作用研究

 目的 探讨阿克替苷(acteoside )的抗肿瘤作用.方法 采用MTT法检测阿克替苷对体外培养的人肿瘤细胞增殖的抑制作用;体内试验采用小鼠H22肝癌,小鼠Lewis肺癌接种小鼠,连续给药14 d,计算抑瘤率.结果 阿克替苷在25～100 μg/ml剂量范围内对肿瘤细胞均有抑制作用;体内试验结果显示小鼠分别灌胃给予2.5、5、10 mg/kg 3个剂量的阿克替苷,对接种的H22细胞株的抑瘤率为17.18%～70.28%,对接种的Lewis肺癌细胞株的抑瘤率为16.9%～55.3%.结论 阿克替苷对体外培养的人肿瘤细胞和接种的小鼠肿瘤具有明显抑制作用.

The antitumor effect of acteoside in vivo and in vitro

Objective To study the antitumor effect of acteoside in vivo and in vitro.Methods The inhibitory effect of acteoside on human tumor cells proliferation was assayed by MTT method.Transplant tumor models of H22 and Lewis were used.After transplantation,different doses of phlomiol were given to the mice for 14 days.The inhibitory rates were calculated.Results The inhibitory rates of acteoside(IP 2.5、5、10 mg/kg) were 17.18%～70.28%and 16.9%～55.3%in tumor- bearing mice with H22 and Lewis respectively.Conclusions Acteoside could inhibit the proliferation of tumor cells in vitro and present antitumor effect on the tumor- bearing mice.