| 秀丽隐杆线虫双突变株系hif-1（ia04）;glb-13（tm2825）的制备与鉴定 |
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| 引用本文： | 石锦平,任长虹,李媛,张成岗.秀丽隐杆线虫双突变株系hif-1（ia04）;glb-13（tm2825）的制备与鉴定[J].军事医学科学院院刊,2011,35(4):253-257,289. |
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| 作者姓名： | 石锦平 任长虹 李媛 张成岗 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所,蛋白质组学国家重点实验室,北京,100850 |
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| 基金项目： | 国家973计划项目，国家科技重大专项课题，国家自然科学基金项目，中国博士后科学基金特别资助基金 |
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| 摘 要： | 目的以秀丽隐杆线虫为模式生物研究珠蛋白（globin）13（glb-13）的生理作用,以回交2次的glb-13（tm2825）突变株系和hif-1（ia04）构建线虫双突变株系hif-1（ia04）;glb-13（tm2825）。方法 glb-13（tm2825）与野生型N2株系交配获得glb-13（tm2825）的雄虫,再与N2的雌雄同体线虫进行交配,回交2次后,通过单只线虫基因组PCR法鉴定出交配后的纯合体。使用hif-1（ia04）和野生型线虫N2进行交配得到hif-1（ia04）的雄虫,然后再与glb-13（tm2825）雌雄同体线虫交配,通过单只线虫基因组PCR法鉴定出交配后的纯合体。结果获得hif-1（ia04）;glb-13（tm2825）双突变株系。结论通过线虫交配回交可以使突变株系线虫保持稳定性状,而突变株系之间的交配可以制备出双突变株系,为深入开展glb-13的功能研究奠定了物质基础。
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| 关 键 词： | 秀丽隐杆线虫 交配 回交 突变 珠蛋白 两性畸形 活性氧类 低氧诱导因子1 |
Construction and characterization of double mutant strain hif-1(ia04);glb-13(tm2825) in C. elegans |
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| SHI Jin-ping,REN Chang-hong,LI Yuan,ZHANG Cheng-gang.Construction and characterization of double mutant strain hif-1(ia04);glb-13(tm2825) in C. elegans[J].Bulletin of the Academy of Military Medical Sciences,2011,35(4):253-257,289. |
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| Authors: | SHI Jin-ping REN Chang-hong LI Yuan ZHANG Cheng-gang |
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| Institution: | SHI Jin-ping,REN Chang-hong,LI Yuan,ZHANG Cheng-gang(State Key Laboratory of Proteomics,Beijing Institute of Radiation Medcine,Beijing 100850,China) |
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| Abstract: | Objective To investigate the physiological function of the glb-13 gene,to backcross glb-13(tm2825) and to construct double mutant strain hif-1(ia04);glb-13(tm2825) in Caenorhabditis elegans.Methods The glb-13(tm2825) mutant male strain was obtained by mating the glb-13(tm2825) mutant hermaphrodite with the male N2 strain,and then with the N2 hermaphrodite.It was then backcrossed twice,and the homozygote was identified by single C.elegans genome PCR.The male hif-1(ia04) mutant strain,which was obtained by ma... |
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| Keywords: | C elegans mating backcross mutation globin hermaphroditism ROS HIF-1 |
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