HPLC法测定倍他米松尿素乳膏中倍他米松和羟苯乙酯的含量

目的建立测定倍他米松尿素乳膏中倍他米松和羟苯乙酯含量的方法。方法色谱柱：PLATISILTMODS（250 mm×4.6 mm,5μm）,流动相：乙腈-水（30∶70）,检测波长：240 nm,流速：1.0 ml·min-1,柱温：45℃。结果倍他米松、羟苯乙酯分别在3.94~23.64μg·ml-1（r=0.9999）、4.78~28.68μg·ml-1（r=1.0000）范围内线性关系良好;回收率、RSD分别为倍他米松99.7%、0.92%,羟苯乙酯100.4%、0.40%。结论本方法简便、准确、重复性好,可用于该制剂中倍他米松和羟苯乙酯的含量测定。

Determination of Beclomethasone and Ethylparaben in Beclometasone and Urea Cream by HPLC

Objective To establish an HPLC method for content determination of beclomethasone and ethylparaben in Beclometasone and Urea Cream.Methods HPLC analysis was carried out on Diamonsil C18column（250 mm × 4.6 mm,5 μm） and with C2H3N-H2O（30∶ 70） as the mobile phase.The detection wavelength was 240 nm,the flow rate was 1.0 ml·min-1and the column temperature was 45 ℃.Results Beclomethasone showed a good linear relationship in the range of 3.94-23.64 μg·ml-1（r = 0.9999）,and ethylparaben in the range of 4.78-28.68 μg·ml-1（r = 1.0000）.The recovery and RSD of beclomethasone and ethylparaben in Beclometasone and Urea Cream were 99.7%,0.92%,100.4% and 0.40%,respectively.Conclusion This method is simple,accurate and repeatable,which is suitable for content determination of beclomethasone and ethylparaben in Beclometasone and Urea Cream.