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| HPLC法测定宁坤养血丸中丹酚酸B的含量 | |
| 引用本文： | 胡丹,包利苹,张兆静,曹红,水彩红,姜春来.HPLC法测定宁坤养血丸中丹酚酸B的含量[J].解放军药学学报,2008,24(1):82-83. |
| 作者姓名： | 胡丹 包利苹 张兆静 曹红 水彩红 姜春来 |
| 作者单位： | 1. 总后勤部卫生部药品仪器检验所,北京,100071 2. 解放军92212部队,卫生处,山东,青岛,266002 3. 解放军第255医院,药械科,河北,唐山,063000 |
| 摘 要： | 目的建立宁坤养血丸中丹酚酸B的含量测定方法。方法采用高效液相色谱法。色谱柱为Agilent Extend-18（250mm×4．6mm，5μm）；流动相为乙腈-1％甲酸水溶液（24：76）；流速为1．0ml／min，检测波长286nm。结果丹酚酸B含量在0．0832—4．16μg范围内，线性关系良好（r=0．9999），平均回收率为104．26％，RSD为1．4％（n=6）。结论结果表明，该方法简便、准确，重现性好。 |
| 关 键 词： | 宁坤养血丸 丹酚酸B HPLC |
| 文章编号： | 1008-9926(2008)01-0082-02 |
| 修稿时间： | 2007年5月18日 |
Determination of Salvianolic Acid B in Ningkun Yangxue Wan by HPLC |
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| HU Dan,BAO Li-Ping,ZHANG Zhao-Jing,CAO Hong,SHUI Cai-Hong,JIANG Chun-Lai.Determination of Salvianolic Acid B in Ningkun Yangxue Wan by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2008,24(1):82-83. | |
| Authors: | HU Dan BAO Li-Ping ZHANG Zhao-Jing CAO Hong SHUI Cai-Hong JIANG Chun-Lai |
| Institution: | HU Dan, BAO Li-Ping, ZHANG Zhao-Jing, CAO Hong, SHUI Cai-Hong, JIANG Chun-Lai(1Institute for Drug and Instrument Control,Health Dept GLD of PLA, Beijing 100071 China;2Health Department of Unit 92212 of PLA, Qingdao 266002, Shandong China;3Department of Pharmacy,255th Hospital of PLA,Tangshan 063000 ; Hebei China) |
| Abstract: | Aim To establish a method for the determinatian of salvianolic acid B in Ningkun Yangxun Wan by HPLC. Methods An external standard method by HPLC with Agilent Extend-18 （250mm×4.6mm,5μm）column was adopted. The mobile phase was acetonitrile-1% formic acid（24： 76）and flow rate was 1.0ml/min. The UV detection wavelength was 286nm. Results The linear range was 0. 083 2 - 4.16 μg（ r = 0.999 9 ）. The average recovery was 104.26% and RSD was 1.4% （ n = 6）. Conclusion The method is simple and accurate, which can be adoptable for quantitative analysis of salvianolic acid B in Ningkun Yangxue Wan. |
| Keywords: | Ningkun Yangxue Wan Salvianolic acid B HPLC |
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