The usefulness of measuring n-butyric acid concentration as a new indicator of blood decomposition in forensic autopsy |
| |
| Institution: | 1. Department of Forensic Medicine, Graduate School of Medicine, Hokkaido University, N15W7 Kita-ku, Sapporo, Hokkaido 060-8638, Japan;2. Center for Cause of Death Investigation, Graduate School of Medicine, Hokkaido University, N15W7 Kita-ku, Sapporo, Hokkaido 060-8638, Japan;3. Center for Medical Education and International Relations, Faculty of Medicine, Hokkaido University, N15W7 Kita-ku, Sapporo, Hokkaido 060-8638, Japan;4. 1st Regional Coast Guard Headquarters, 5-2, Minatomachi, Otaru, Hokkaido 047-8560, Japan;5. Faculty of Health Sciences, Hokkaido University, N12W5, Kita-ku, Sapporo, Hokkaido 060-0812, Japan;1. Posgrado de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Ciudad Universitaria Cercado de Lima, Lima, Peru;2. Unidad de Biología Molecular y de Genética (UNBIMOG), Instituto de Medicina Legal y Ciencias Forenses del Ministerio Público, Lima, Peru;3. Center for Human Identification, University of North Texas Health Science Center, Ft Worth, TX 76107, USA;4. Consultant, Christiansburg, VA 24073, USA;5. Universidad Privada Peruano Alemana, Lima, Peru;6. Escuela de Posgrado, Universidad Privada Norbert Wiener, Lima, Peru;1. Institute of Forensic Medicine, University Hospital Ostrava, 17. listopadu 1790/5, Ostrava CZ-70852, Czech Republic;2. Department of Pathology, Faculty of Medicine, Ostrava University, CZ-703 00 Ostrava, Czech Republic;3. Department of English and American Studies , Faculty of Arts, Palacký University Olomouc, CZ-779 00 Olomouc, Czech Republic;4. The Fingerland Department of Pathology, Faculty of Medicine in Hradec Kralove, Charles University, CZ-500 03 Hradec Kralove, Czech Republic;1. Centre for DNA Identification, Institute of Biotechnology, Vietnam Academy of Science and Technology, Co Nhue, Hanoi 100000, Viet Nam;2. Department of Forensic Science, People’s Police Academy, Hanoi, Viet Nam;3. National Key Laboratory of Gene Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Viet Nam;1. Department of Environmental Sciences and Natural Resources, University of Alicante, PO Box 99, E-03080 Alicante, Spain;2. Department of Medical Microbiology and Parasitology, Faculty of Medicine, Sungai Buloh Campus, Universiti Teknologi MARA, Sungai Buloh, 47000 Selangor, Malaysia;3. Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, England;1. Forensic Medicine Unit, Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, ASST Spedali Civili of Brescia, Piazzale Spedali Civili 1, 25123 Brescia, Italy;2. Department of Medical and Surgical Specialities, Radiological Sciences and Public Health, University of Brescia, Viale Europa 11, 25123 Brescia, Italy;3. Forensic Pathologist, Fondazione Poliambulanza Istituto Ospedaliero, via Bissolati 57, 25124 Brescia, Italy;4. Department of Molecular and Translational Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy;1. Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041 Sichuan, PR China;2. Department of Forensic Pathology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, PR China;3. College of Computer Science, Sichuan University, Chengdu 610065 Sichuan, PR China;4. Department of Radiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China;5. Department of Radiology, The People’s Hospital of Leshan, Leshan, Sichuan 614000, PR China |
| |
| Abstract: | In forensic medicine, although various alcohols have been reported as indicators of decomposition in collected blood, no studies have examined short-chain fatty acids as indicators. In this study, the blood n-butyric acid concentration was quantified, and the association between n-butyric acid and decomposition was investigated to determine whether the detection of n-butyric acid could be a new indicator of decomposition. Among the forensic autopsies performed from 2016 to 2018 in our laboratory, the cases were divided into decomposed (n = 20) and non-decomposed (n = 20) groups based on macroscopic findings. Blood samples collected at the time of autopsy were derivatized with 3-nitrophenylhydrazine hydrochloride after solid-phase extraction. The n-butyric acid concentration was measured using liquid chromatography–tandem mass spectrometry. In addition, ethanol and n-propanol were measured using a gas chromatography-flame ionization detector. There was a significant difference (p < 0.01) in the concentrations of n-butyric acid between the decomposed and non-decomposed groups (0.343 ± 0.259 0.030–0.973] and 0.003 ± 0.002 0.001–0.007] mg/mL, respectively). In the decomposed group, n-butyric acid was detected at high concentrations, even in cases where n-propanol was low. These results suggest that n-butyric acid is more likely to be an indicator of blood decomposition than n-propanol. |
| |
| Keywords: | Postmortem decomposition Liquid chromatography–tandem mass spectrometry Forensic autopsy Postmortem production |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
