Immunohistochemical analysis of von Willebrand factor expression in myocardial tissues from autopsies of patients with ischemic heart disease |
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| Institution: | 1. Division of Legal Medicine, Department of Community Medicine and Social Healthcare Science, Kobe University Graduate School of Medicine, Kobe, Japan;2. Forensic Science Laboratory, Hyogo Prefectural Police Headquarters, Kobe, Japan;3. Department of Legal Medicine, Ehime University Graduate School of Medicine, Toon, Japan;1. Department of Maxillofacial Radiology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan;2. Department of Epidemiology and Preventive Medicine, Field of Human and Environmental Sciences, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan;3. Department of Toxicology and Cancer Biology, 454 Bosomworth HSRB, University of Kentucky College of Medicine, Lexington, KY 40536, USA;4. Department of Gross Anatomy, Field of Neurology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan;5. Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, Japan;1. DNA Fingerprinting Unit, State Forensic Science Laboratory, Department of Home (Police), Govt. of MP, Sagar 470001, India;2. DNA Division, State Forensic Science Laboratory, Rajasthan, Jaipur 302016, India;3. Institute of Forensic Science and Criminology, Bundelkhand University, Jhansi 284128, UP, India;1. School of Forensic Medicine, Kunming Medical University, Kunming, Yunnan Province, People’s Republic of China;2. Judicial Expertise Center of Kunming Medical University, Kunming, Yunnan Province, People’s Republic of China;1. Department of Law, Institute of Legal Medicine (AgEstimation Project), University of Macerata, Macerata, Italy;2. Postgraduate Program in Dentistry, Federal University of Paraíba, Campus I, João Pessoa, PB 58051900, Brazil;3. Department of Radiological Sciences, Ospedali Riuniti, Marche Polytechnic University, Ancona, Italy;4. School of Law, Legal Medicine, University of Camerino, Camerino, Italy;5. Department of Forensic Medicine, IM Sechenov First Moscow State Medical University, Moscow, Russian Federation;6. AgEstimation Project, Department of Forensic Medicine, University of Molise, Italy |
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| Abstract: | von Willebrand factor (VWF) plays a crucial role in hemostasis and thrombosis. VWF is involved in platelet attachment to the subendothelium, serving as a carrier protein for coagulation factor VIII. In this study, myocardial tissues from deceased patients with ischemic heart disease and a mouse model of acute myocardial infarction were subjected to immunohistochemistry to determine VWF expression. We examined 28 neutral formalin-fixed, paraffin-embedded myocardial tissue samples obtained from the autopsies of patients who were diagnosed with ischemic heart disease within 48 h postmortem. Most myocardial cells were negative for VWF, although some cells showed nonspecific positivity. Elevated VWF expression was observed around myocardial cells undergoing remodeling, suggesting that endothelial proliferation occurred at these sites. In contrast, completely fibrotic myocardial foci did not show upregulated VWF expression. Positivity in fibrin deposition and hemorrhagic sites was observed. The same VWF expression characteristics as those observed in the human samples were observed in the mouse model. VWF immunostaining as an endothelial marker may be a useful supplementation to conventional staining techniques that are currently used in the diagnosis of ischemic heart disease in terms of examining the timing of myocardial remodeling in detail and highlighting the remodeling process. |
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| Keywords: | Von Willebrand factor Immunohistochemistry Endothelial marker Myocardial remodeling Forensic pathology |
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