Evaluation of the MHSeqTyper47 kit for forensically challenging DNA samples |
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| Institution: | 1. Beijing Institute of Microbiology and Epidemiology, 27 Taiping Road, Beijing 100850, PR China;2. Department of Pathology and Forensic Medicine, College of Medicine, Yanbian University, No. 977 Park Road, Jilin 133002, PR China;1. Dipartimento di Biologia e Biotecnologie \"Charles Darwin\", Sapienza Università di Roma, Rome, Italy;2. Reparto Carabinieri Investigazioni Scientifiche di Roma – Sezione di Biologia, Rome, Italy;3. Istituto di Biologia e Patologia Molecolari, Consiglio Nazionale delle Ricerche, Rome, Italy;4. Department of Forensic Sciences, Naïf Arab University for Security Sciences, Riyadh, Kingdom of Saudi Arabia;1. School of Forensic Science and Technology, Criminal Investigation Police University of China, Shenyang, Liaoning 110854, PR China;2. School of Public Security Information Technology and Intelligence, Criminal Investigation Police University of China, Shenyang, Liaoning 110854, PR China;3. DNA Laboratory of Forensic Science Center, Shenyang Public Security Bureau, Shenyang, Liaoning 110002, PR China;4. Heyuan Public Security Forensic Science Center, Heyuan, Guangdong 517000, PR China;5. Thermo Fisher Scientific (China) Co., Ltd., Shanghai 200120, PR China;1. University Ulm, Institute of Legal Medicine, Albert-Einstein-Allee 23, Ulm 89081, Germany;2. Ludwig-Maximilians University Munich, Geschwister-Scholl-Platz 1, München 80539, Germany;1. Division of Biological Traces, Netherlands Forensic Institute, The Hague, the Netherlands;2. Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands;1. Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, China;2. Department of Forensic Biology, West China School of Basic Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan, China |
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| Abstract: | Microhaplotypes have been highly regarded for forensic mixture DNA deconvolution because they do not experience interference from stutters in the same way as short tandem repeat markers, and they tend to be more polymorphic than single nucleotide polymorphism markers. However, forensic microhaplotype kits have not been reported. The MHSeqTyper47 kit genotypes 47 microhaplotype loci. In this study, MiSeq FGx sequencing metrics for MHSeqTyper47 were presented, and the genotyping accuracy of this kit was examined. The sensitivity of MHSeqTyper47 reached 62.5 pg, and full genotyping results were obtained from degraded DNA samples with degradation indexes ≤ 3.00. Full genotypes were obtained in the presence of 100 ng/μL tannin, 50 μM heme, 25 ng/μL humic acid, and 1.25 μg/μL indigo dye. In DNA mixture studies, a minimum of 31 loci of the minor contributor were correctly genotyped at 1:99 or 99:1 mixing ratios, with the cumulative random matching probability of these loci reaching 4.54 × 10?25. Mixing ratios could be reliably predicted from two-donor DNA mixtures based on the loci with four called alleles. Taken together, these data showed that the MHSeqTyper47 kit was effective for forensically challenging DNA analysis. |
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| Keywords: | Forensic genetics Microhaplotype DNA mixture |
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