复发性卵巢癌血清差异表达蛋白分析

目的探讨血清差异表达蛋白对提高复发性卵巢癌的诊断意义。方法采用弱阳离子（WCX）磁珠纯化试剂盒和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）技术,对21例复发性卵巢癌和18例完全临床缓解期卵巢癌患者（对照组）的血清标本进行检测,筛选出复发性卵巢癌患者与对照组血清中的差异表达质谱峰,并建立诊断模型。结果在Mr1000～12000区段,发现复发性卵巢癌与对照组间差异表达蛋白峰10个（P〈0.05）。其中Mr1944、1980、2080、2661、2993、4450、4659、5359的蛋白峰显著上调,Mr1897、7868则显著下调。结论 MALDI-TOF-MS结合磁珠技术能直接检测出复发性卵巢癌患者血清差异表达蛋白,对提高复发性卵巢癌诊断的敏感性和特异性具有一定的临床意义。

Analysis of diferential expressed protein from recurrent ovarian cancer serum

Objective To study the value of the diferential expressed proteins from recurrent ovarian cancer serum for early diagnosis of recurrent ovarian cancer.Methods WCX kit （Bruker Daltonics GraBH）and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry technology were used to detect serum samples from 21 patients with recurrent ovarian cancer and 18 patients with complete clinical remission.Results In the mass range from 1 000 to 12 000 Da,10 diferential expressed protein peaks were screened from recurrent ovarian cancer serum .Among them ,1944,1980,2080,2661,2993,4450,4659,5359 Da protein expressions were increased significantly,and 1897,7868 Da protein expressions were decreased significantly.Conclusion Combination of MALDI-TOF-MS and WCX kit technology can directly screen the diferrential expressed protein from recurrent ovarian cancer serum.They have clinical significance for enhancement of sensitivity and specificity of ovarian cancer diagnosis.