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腺病毒介导的p16和p53的联合应用对胆管癌细胞QBC939的生长抑制作用
引用本文:鲁建国,马庆久,要秀,林晨,付明,张雪艳,梁萧,吴旻,黄志强.腺病毒介导的p16和p53的联合应用对胆管癌细胞QBC939的生长抑制作用[J].解放军医学杂志,2001,26(6):393-395.
作者姓名:鲁建国  马庆久  要秀  林晨  付明  张雪艳  梁萧  吴旻  黄志强
作者单位:1. 第四军医大学唐都医院,
2. 中国医学科学院、中国协和医科大学肿瘤研究所
3. 解放军总医院普外研究所
基金项目:863高科学技术发展资助课题!(编号Z2 0 0 1 0 2 )
摘    要:为研究p16和p53基因的联合应用对胆管癌细胞的作用,将重组体腺病毒p16、p53、p16联合p53转移到人胆管癌细胞QBC939,对p16、p53基因的表达,细胞的生长抑制及机制进行了分析。RT-PCR显示在胆管癌QBC939细胞系中p16呈低表达,p53不表达,重组体腺病毒能介导p16和p53等外源基因在胆管癌QBC939细胞系中高效表达,两者联合应用能明显抑制QBC939细胞的生长和集落形成。流式细胞计数证实其能诱导,QBC939细胞发生明显半调并导致其发生G1期阻滞,本研究显示p16及p53基因通过诱导肿瘤细胞凋亡及G1期阻滞在肿瘤的基因治疗方面发挥作用,两者联合应用具有协同作用。

关 键 词:胆管癌  p16基因  p53基因  腺病毒  基因治疗
修稿时间:2000年3月23日

INHIBITORY EFFECTS OF HUMAN CHOLANGIOCARCINOMA CELL LINE BY RECOMBINANT ADENOVIRUSES p16 AND p53 TRANSDUCTION
Abstract:To evaluate the inhibitory effect of recombinant adenoviruses p16 (Ad p16) combined with Ad p53 on human cholangiocarcinoma cell line QBC939 in vitro. Expression of p16 was low and p53 was negative in QBC939 cells. High level of p16 and p53 expressions were observed in QBC939 cell line transfected with Ad p16 and Ad p53. The growth rates of the Ad p16 infected combined with Ad p53 infected QBC939 cells were inhibited, which was higher than those of the Ad p53 or close to Ad p16 transfection. Colony formation in Ad p16 with Ad p53 transfected cells greatly decreased versus Ad p16 transfected or Ad p53 transfected cells. The Ad p16, Ad p53 and Ad p16 with Ad p53 transfected cells manifested apoptosis and G 1 arrest, which were confirmed by the flow cytometry. The suppression effects mediated by expression of the exogenous p16 with p53 in tumor cell resulted mainly from apoptosis and G 1 arrest, which is higher than exogenous p16 or p53.
Keywords:cholangiocarcinoma  genes  p16  genes  p53  adenovirus  gene therapy
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