不同波段电磁辐射致大鼠Sertoli细胞SOX9和WT1表达的影响

目的 探讨不同波段电磁辐射对大鼠睾丸支持细胞(Sertoli细胞)性别决定相关基因SOX9和WT1表达的影响。方法 原代分离3周Wistar大鼠的Sertoli细胞,应用WT1免疫细胞化学染色鉴定细胞纯度;Sertoli细胞经平均功率密度为100 mW/cm²的S波段微波和X波段微波及场强6×10⁴ V/m的电磁脉冲辐射4 min。应用实时RT-PCR和Western blot方法,检测SOX9、WT1的mRNA和蛋白质的变化。结果 3种波段电磁辐射后Sertoli细胞SOX9 mRNA和蛋白表达在辐射后6和12 h及辐射后的6和24 h均见不同程度的降低(F=15.20和4.84, P<0.05;F=8.46和7.47,P<0.05);WT1 mRNA和蛋白表达在辐射后6和12 h及辐射后的24 h出现不同程度降低(F=13.46、5.08和10.26,P<0.05)。结论 3种波段电磁辐射后Sertoli细胞性别决定相关基因(SOX9、WT1)表达呈不同程度的降低。Sertoli细胞SOX9、WT1的变化可能参与了电磁辐射致生殖危害的病理生理过程。

Effect of electromagnetic radiation at different bands on the expressions of SOX9 and WT1 in rat Sertoli cells

Objective To explore the changes of SOX9 and WT1 expressions in rat Sertoli cells irradiated by EMP (electromagnetic pulse), S-HPM (S-band high power microwave) and X-HPM (X-band high power microwave). Methods Primary Sertoli cells were isolated from 3-week-old Wistar rats and its purity was immunocytochemistrically indentified with WT1. After exposure to 6×10⁴ V/m EMP, 100 mW/cm² S-HPM and X-HPM for 4 min respectively, SOX9 and WT1 expressions in Sertoli cells were determined with real-time PCR and Western blot, respectively. Results SOX9 mRNA expression was decreased at 6 and 12 h post-irradiation of three different bands of electromagnetic microwave (F=15.20 and 4.84, P<0.05). SOX9 protein expression was also decreased at 6 and 24 h after irradiation (F=8.46 and 7.47, P<0.05). WT1 mRNA expression was decreased at 6 and 12 h (F=13.46 and 5.08, P<0.05), but its protein expression was decreased only at 24 h post-irradiation (F=10.26, P<0.05). Conclusions Three bands of electromagnetic radiation reduce the expressions of SOX9 and WT1 in rat Sertoli cells, which may provide molecular foundation for genital system hazards induced by microwave radiation.