CXCR4基因转染促进移植dMSCs向放创复合伤大鼠创面分布的研究

目的 探讨CXCR4腺病毒表达载体(Adv-CXCR4)转染的真皮多能干细胞(dMSCs)移植后可否更多地向放创复合伤大鼠创面分布,并加速创面愈合。方法 ³H-TdR标记Adv-CXCR4转染的dMSCs(A组)、绿色荧光蛋白腺病毒表达载体转染的dMSCs(B组)、未转染病毒载体的dMSCs(C组)后移植到放创复合伤大鼠体内,液闪法测量创面中dMSCs的含量。用生物医学图像分析仪测量伤后创面残留面积。结果 从伤后5 d起,A组创面中dMSCs的含量约占移植细胞总量的1.95%～3.85%,明显高于B组和C组(占移植细胞总量的1.07%～1.86%)。从伤后12 d开始,A组创面残留面积明显小于B组和C组。A组创面愈合时间比B组和C组提前约1.5 d。结论 Adv-CXCR4转染的dMSCs在移植后可更多地分布到放创复合伤大鼠的创面,且能更快地促进创面愈合。

CXCR4 gene transfection enhancing the distribution of dMSCs to the wounded skin of rats with combined wound and irradiation injury

Objective To observe whether the transplanted dermal multipotent stem cells (dMSCs) transfected by adenovirus vector of CXCR4 (Adv-CXCR4) can distribute more frequently to the wound of rats with combined wound and irradiation injury. Methods dMSCs transfected by Adv-CXCR4 (group A), or transfected by adenovirus vector of green fluorescent protein (group B), and non-transfected dMSCs were labeled with ³H-TdR and then transplanted into combine-injured rats. The amount of dMSCs in wound were determined by liquid scintillation, and wounds healing process was observed by measuring the remaining wound area. Results From the 5th day after transplantation, the amount of dMSCs in the wound of group A accounted for 1.95%-3.85% of the total transplanted dMSCs, significantly greater than those in group B and group C, which accounted for 1.07%-1.86% of the total transplanted dMSCs. The remaining wound area in group A was smaller than those in group B and group C from day 12 after injury, and the healing time of group A was 1.5 day ahead than group B and group C. Conclusions dMSCs transfected by Adv-CXCR4 distributes more frequently to the wound of combine-injured rats and could accelerate wound healing.