甲状旁腺素对辐射损伤小鼠造血功能的防护作用

目的 探讨重组人甲状旁腺素(parathyroid hormone, PTH)对辐射所致小鼠造血功能损伤的防护作用。方法 雄性C57小鼠60只,经⁶⁰Co γ射线照射后随机分为PTH治疗组和照射对照组。PTH治疗组每天给予PTH 80 μg ·kg^-1 ·d^-1 腹腔注射,对照组给予等量生理盐水。全自动血细胞分析仪检测小鼠外周血细胞数,手工计数骨髓有核细胞数,流式细胞仪分析骨髓中CD34+细胞数,集落细胞培养法观察骨髓粒系造血祖细胞数。结果 在照射后10、15和20 d,PTH治疗组小鼠的外周血红细胞计数显著高于照射对照组(t=6.48、3.66和4.98,P<0.05)。在照射后各时间点外周血白细胞计数显著高于照射对照组(t=6.32、9.19、11.18、7.44和4.42,P<0.05)。在照射后5和30 d血小板计数显著高于照射对照组(t=2.57和3.10,P<0.05)。在照射后各时间点PTH治疗组骨髓有核细胞数均显著高于照射对照组(t=5.80、3.72、17.89、4.90和16.49,P<0.05)。PTH显著增加了照射后小鼠的骨髓粒细胞集落形成单位数和CD34+细胞数(t=16.12、7.82和20.00,P<0.05)。结论 甲状旁腺素对辐射引起的造血损伤具有明显的保护作用。

Protective effects of parathyroid hormone on radiation-induced hematopoietic damage in mice

Objective To study the protective effects of parathyroid hormone (PTH) on radiationinduced hematopoietic damage in mice.Methods A total of sixty male C57 mice were irradiated by 60Co γ-rays to induce hematopoietic injuries,and then the mice were randomly divided into PTH and control group.The PTH-treated group was treated with PTH ( 80 μg· kg- 1 · d - 1 ) intraperitoneally everyday.The control group was given equivalent volume saline.Peripheral blood cell number,bone marrow mononuclear cell number,granulocyte-macrophage colony forming units ( CFU-GM ) and CD34 positive cells in bone marrow were detected.Results With the whole post-irradiation period,the WBC and bone marrow mononuclear cell numbers in PTH-treated mice were significantly higher than those in saline-treated mice (t=6.32,9.19,11.18,7.44 and 4.42,P ＜ 0.05).The RBC numbers in PTH-treated mice were significantly higher than those in control mice at 10 d,15 d and 20 d post-irradiation (t =6.48,3.66 and 4.98,P ＜0.05 ).The PLT numbers in PTH-treated mice were significantly higher than those in control group at 5 and 30 d post-irradiation ( t =2.57 and 3.10,P ＜ 0.05 ).PTH increased CD34 positive cell and CFU-GM numbers in bone marrow after irradiation ( t =16.12,7.82 and 20.00,P ＜ 0.05 ).Conclusions PTH could improve the hematopoietic recovery after irradiation.