放射损伤、烧伤及放烧复合伤后血清成分对培养心肌细胞内向整流性钾通道活动的影响

探讨放射损伤、烧伤与放烧复合伤后血清低分子成分与血清脂质成分对培养心肌细胞内向整流性钾离子通道活动的影响。方法膜片钳单通道方法测定离子通道活动的变化。结果伤后血清成分对正常培养心肌细胞内向整流性钾通道有激活作用。其中，复合伤血清成分的作用更显著，且与单一伤者在作用方式上有所不同。结论伤后血清成分对心肌内向整流性钾通道活动的影响在伤后心脏功能抑制中起一定的作用，在复合伤后心功能抑制加重的复合效应中也可能起一定的作用

Effects of different components of serum after radiation burn and combined radiation burn injury on inward rectifier potassium channel of myocardial cells

Objective \ To study the effects of different components of serum in rats inflicted with radiation, burn and combined radiation burn injury on inward rectifier potassium channel of cultured myocardial cells. Method\ Using patch clamp method to study the action of single ion channel. Results\ The low molecular and lipid components of serum after different injuries models could all activate the inward rectifier potassium channel in cultured myocardial cells. The components of serum after combined radiation burn injury showed the most significant effect, and the way of this effect was different from that from single injury. Conclusion\ The serum components post injury altered the electric characteristic of myocardial cells, which may play a role in the combined effect of depressed cardiac function after combined radiation burn injury.