不同质量浓度高碘饮食对小鼠甲状腺摄锝功能的影响

目的观察不同质量浓度高碘饮食对小鼠甲状腺摄锝功能的影响。方法采用完全随机设计方法将288只实验小鼠分为0～7共8个组，其中0组为对照组，正常饮食；1～7组为实验组，分别给予不同质量浓度高碘饮食，含碘量依次为：50，100，150，200，250，500和5000μg／L，喂养1周后，每只小鼠腹腔内注射^99Tc^mO4^-3.7MBq，每组均分别于注射后10，20，25，30，45和60min处死小鼠并取甲状腺，测量其每克组织百分注射剂量率（％ID／g）。采用Dunnett-t检验对数据进行比较，观察高碘饮食对小鼠甲状腺摄锝功能的影响。结果与对照组相比，当饮食中碘质量浓度为150μg/L时，小鼠甲状腺摄锝功能在各个时间点上开始受到明显抑制t0.3（10min=3．13，t0.3（20min）=4．54，t0.3（25min）=3．86，t0.3（30min）=3．69，t0.3（45min）=2．63，t0.3（60min）=2．82，P均〈0.05]。随着碘质量浓度增高，小鼠甲状腺摄锝功能逐渐降低。结论碘离子质量浓度≥150μg／L时，小鼠甲状腺摄锝功能明显受抑制。

Effects of different amounts of iodine on 99TcmO4- uptake of thyroid in mice

Objective The aim of this study was to observe the dose-dependent effect of iodine on thyroid uptake of 99TcmO4- in mice. Methods Kunming mice were randomly divided into 8 groups. One control group fed with water, the others were dose-dependent iodine groups fed with various KIO3 concentration of 50, 100, 150,200,250,500, 5000 ug/L, respectively for 7 d. Thyroid weight and thyroid uptake percentage activity of injected dose per gram of tissue (% ID/g) of 99TcmO4- was measured at different times. Dunnett-t test was used for statistically analysis. Results Significantly lower uptake of 99TcmO4- in mice thyroid was noted with time when KIO3 concentration was equal or above than 150 ug/Lt0,3(10min)= 3.13, t0,3(20 min)=4.54, t0,3(25min)=3.86, t0,3(30min)=3.69, t0,3(45min)=2.63, t0,3(60 min)=2.82, all P< 0.05]. Conclusion The cutoff of KIO3 concentration that would influence the uptake of 99TcmO4- in mice thyroid was equal or above than 150 ug/L.