实验肿瘤局部照射动物模型的改进

目的对局部照射肿瘤实验动物模型进行改进以提高肿瘤放疗增敏药物增敏实验结果的可靠性。方法分别在小鼠腿上皮下（传统方法）和小鼠脚背皮下（改进方法）接种肝癌H22细胞，使其生长成实体瘤；用5Gyγ射线照射肿瘤后，每隔1d测量肿瘤的长、宽、高并计算肿瘤体积，连续观察24d。结果将肿瘤接种于小鼠腿上皮下时，照射组的肿瘤体积和质量与对照组之间的差异无统计学意义（t=0．55、0．70，P均〉0．05）；而将肿瘤接种于脚背皮下时，照射组的肿瘤体积和质量均明显低于对照组（t=2．25，P〈0．05；t=3．14，P〈0．01）。结论肿瘤接种于小鼠脚背皮下的方法，操作简单、方便、易行且实验结果准确、可靠。

Improvement on the animal model of local irradiation for experimental tumors

Objective To improve the reliability of experimental outcome in radio-sensitive drug research by improving the animal model of locally irradiated tumor. Methods Liver cancer cells H22 were implanted into legs and insteps subcutaneously in mice, respectively, and made them grow into solid tumors. Then the tumors were treated with the γ ray of 137Cs at the dosage of 5 Gy. Measured length, width and height of tumors once per day, and calculated volume of tumors, observed for 24 days. Results Volume and weight of the irradiated tumor inoculated into legs were no obvious difference （t=0.55, 0.70, both P〉0.05）. But volume and weight of the irradiated tumors inoculated subcutaneously into insteps of the tested mice were obviously lower than that of control mice （t=2.25, P〈0.05 in volume; t=3.14, P〈0.01 in weight）. Conclusion The insteps subcutaneous approach of tumors in mice is a more simple, convenient and practicable operation, and will make the testing result more accurate and reliabe.