脂蛋白脂酶基因HindⅢ多态性与体质量指数及胰岛素抵抗关系的研究

目的探讨脂蛋白脂酶基因变化与2型糖尿病胰岛素抵抗的关系。方法采用聚合酶链式反应法对122名大连籍2型糖尿病病人和56名同期非糖尿病体检者,脂蛋白脂酶基因第8号内含子H indⅢ酶切点进行多态性分析,同时以放免法测定以上两组人群空腹胰岛素及血糖水平。结果两组基因频率无显著差异,但是H+H+组体质量指数、胰岛素抵抗指数显著高于非H+H+组。结论脂蛋白脂酶基因H indⅢ酶切点多态性与2型糖尿病胰岛素抵抗关联。

Investigation into the Relation between Hind Ⅲ Polymorphism of Lipoprotein Lipase Gene and Body Mass Index or Insulin Resistance

Objective To investigate into the relation between the alteration of lipoprotein lipase gene and insulin resistance of type Ⅱ diabetes.Methods Polymorphic analysis on HindⅢ restricted site of No.8 intron of lipoprotein lipase gene was conducted via polymerase chain reaction(PCR) on 122 Dalian originated patients with type Ⅱ diabetes and 56 subjects without diabetes who accepted physical examination,and meanwhile,their fasting insulin and glucose levels were measured via radioimmunoassay(RIA).Results There was no significant difference in gene frequency between the two groups,but the body mass and insulin resistance indexes of those with H~+H~+ were obviously higher than that of those with H~-H.Conclusion Polymorphism of HindⅢ restricted site of lipoprotein lipase gene is relative to insulin resistance of type Ⅱ diabetes.