创伤小鼠反抑制T细胞的变化

研究创伤对反抑制细胞的影响，方法采用小鼠截肢伤模型，利用长柔毛野豌豆凝集素分离取Ｔｃｓ细胞，以直接免疫荧光法检测Ｔｃｓ细胞数目；于正常Ｔ淋转，白介素２、ＩＬ－２受体检测系统中测定Ｔｃｓ细胞的反抑制活性变化；并作光阄，透射电镜观察。

Changes of Contrasuppressor T Cells in Traumatized Mice

Objective Effect of trauma on contrasuppressor T cells (Tcs) was studied. Methods A murine amputation injury model was used. Tcs were separated using the vicia villosa lective (VVL). Tcs number was measured with direct immunofluorescence method. Contrasuppressive activity of Tcs was determined in the measuring system of normal T lymphocyte transformation. Interleukin-2 (IL-2), IL-2 receptor (IL-2R), and morphological structure of Tcs were observed under light microscope and penetrating electron microscope. Results The number of Tcs from the spleen and thymocyte was decreased after trauma while no significant change was noticed in mesenteric lymph nodes. Contrasuppressive activity of Tcs was decreased on 1st day after trauma and became more obvious on day 4 to 10. This change did not yet return to normal on day 14. The morphological structure of Tcs was not seen abnormal under light microscope, but under penetrating electron microscope, bleb formation was seen in Tcs cytoplasm, pseudounclusion in nucleus, and rarely cytolysis with naked nucleus were observed on day 14 posttrauma. Conclusions Trauma may result in changes of Tcs in number, functions and morphological structure at various degrees.