Anionic polymers and 10 nm Fe3O4@UA wound dressings support human foetal stem cells normal development and exhibit great antimicrobial properties |
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Authors: | Alexandru Mihai Grumezescu Alina Maria Holban Ecaterina Andronescu George Dan Mogo?anu Bogdan Stefan Vasile Mariana Carmen Chifiriuc Veronica Lazar Eugen Andrei Andrei Constantinescu Horia Maniu |
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Institution: | 1. University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street No. 1-7, 011061 Bucharest, Romania;2. University of Bucharest, Faculty of Biology, Microbiology Department, Aleea Portocalelor, No. 1-3, 060101 Bucharest, Romania;3. Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rare? Street, 200349 Craiova, Romania;4. Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology “Nicolae Simionescu” (ICBP), Bucharest, Romania |
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Abstract: | The aims of this study were the development, characterization and bioevaluation of a novel biocompatible, resorbable and bio-active wound dressing prototype, based on anionic polymers (sodium alginate – AlgNa, carboximethylcellulose – CMC) and magnetic nanoparticles loaded with usnic acid (Fe3O4@UA). The antimicrobial activity was tested against Staphylococcus aureus grown in biofilms. The biocompatibility testing model included an endothelial cell line from human umbilical vein and human foetal progenitor cells derived from the amniotic fluid, that express a wide spectrum of surface molecules involved in different vascular functions and inflammatory response, and may be used as skin regenerative support. The obtained results demonstrated that CMC/Fe3O4@UA and AlgNa/Fe3O4@UA are exhibiting structural and functional properties that recommend them for further applications in the biomedical field. They could be used alone or coated with different bio-active compounds, such as Fe3O4@UA, for the development of novel, multifunctional porous materials used in tissues regeneration, as antimicrobial substances releasing devices, providing also a mechanical support for the eukaryotic cells adhesion, and exhibiting the advantage of low cytotoxicity on human progenitor cells. The great antimicrobial properties exhibited by the newly synthesized nano-bioactive coatings are recommending them as successful candidates for improving the implanted devices surfaces used in regenerative medicine. |
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Keywords: | Magnetite nanoparticles Wound dressing Regenerative medicine Sodium alginate Carboximethylcellulose Human foetal progenitor cells |
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