蟾毒灵对人膀胱癌T24细胞增殖和凋亡的影响

摘要目的：研究蟾毒灵对人膀胱癌T24细胞增殖和凋亡的影响，初步探讨其在膀胱癌中的抗肿瘤机制。方法：采用MTT法测定蟾毒灵对人膀胱癌T24细胞增殖的影响；流式细胞术（FCM）检测蟾毒灵对癌细胞凋亡和细胞周期的影响；RT—PCR法检测癌细胞周期蛋白D1（CyclinDl）基因表达情况。结果：蟾毒灵可抑制人膀胱癌T24细胞的增殖，并呈现出时间和浓度依赖性；蟾毒灵能诱导T24细胞凋亡；20nmol／L浓度的蟾毒灵预处理膀胱癌T24细胞后，与对照组（36．42％）进行比较，可分别在12h（69．76%）和24h（88．14%）显著诱导细胞G0／G1期阻滞（P〈0．05），且可降低膀胱癌T24细胞的CyclinDlmRNA表达水平。结论：蟾毒灵能显著抑制人膀胱癌T24细胞增殖，诱导T24细胞G0／G1周期阻滞，下调人膀胱癌T24细胞CyclinDl基因表达。

Effect of bufalin on the proliferation and apoptosis of human bladder cancer T24 cells

ABSTRACT AIM： To investigate the effect of bufalin on the proliferation of human bladder cancer T24 cells and explore the underlying anti- cancer mechanisms of bufalin. METHODS： The proliferation-inhibiting effect of bufalin on T24 cells was measured by methyl thiazole tetrazoli- um （MTT）colorimetric assay the cell cycle dis- tribution and apoptosis were detected by flow cytometry （ FCM ） the expression of CyclinD1 was detected by RT-PCR. RESULTS：Bufalin in- hibited the proliferation of T24 cells in a time- and dose-dependent manner bufalin could in- duce apoptosis of T24 cell exposure of T24 cells to 20 nmol/L of bufalin led to significant induc-tion of G0/G1 growth arrest at both 12 h （69.76%） and 24 h （88.14%）, compared with negative control cells （36.42%）. The mRNA levels of CyclinD1 in T24 cells was markedly de- creased after treatment with bufalin. CONCLU- SION： Bufalin could inhibit the proliferation and induce apoptosis of T24 cells. Bufalin could in- duce T24 G0/G1 growth arrest and decrease the mRNA levels of CyclinD1.