蝎毒结肠靶向小球对幼鼠慢性内脏痛的抑制作用

目的:研究蝎毒结肠靶向小球对幼鼠慢性内脏痛的抑制作用。方法:选用新生SD大鼠,出生后8～14d内,每天固定时间给予一次60mm Hg结直肠扩张刺激,建立慢性内脏痛模型,对照大鼠除了不行结直肠扩张外,其他情况同模型大鼠。实验分组:对照组、对照空白小球组、对照蝎毒小球组、模型组、模型空白小球组、模型蝎毒小球低剂量组、模型蝎毒小球中剂量组、模型蝎毒小球高剂量组。大鼠第28天开始胃肠给药,连续给予蝎毒靶向制剂14d后,采用腹外斜肌放电来评估肠道痛觉的敏感性;进而采用离体脑片场电位的记录方法,观察慢性内脏痛幼鼠海马CAl区场电位LTP的变化。结果:模型幼鼠腹外斜肌放电明显增强,服用蝎毒靶向小球后,腹外斜肌放电幅值显著减少(P<0.05);记录离体海马场电位LTP显示,模型幼鼠场电位LTP较对照幼鼠的幅值显著增加(P<0.05),同时蝎毒结肠靶向小球可显著降低模型幼鼠海马场电位LTP的幅值(P<0.05),而对对照幼鼠的作用无统计学差异(P>0.05)。结论:蝎毒结肠靶向小球可抑制幼鼠慢性内脏痛觉敏感性。

Inhibitory effect of colon-targeted ball of scorpion venom on young rats with chronic visceral hypersensitivity

AIM： To explore the inhibitory effect of colon-targeted ball of scorpion venom on model young rats with chronic visceral hypersensitivity.METHODS： Neonatal male Sprague-Dawley（SD）rats were used as study subject.Model rats were received 60 mm Hg colon irritation（CI） for a minute once daily from post neonatal days 8 to 14;control rats were received the same processes except CI.The experiment groups were divided into control group,control blank ball group,control scorpion venom ball group,model group,model blank ball group,model scorpion venom ball group（low）,model scorpion venom ball group（medium） and model scorpion venom ball group（high）.Colon-targeted ball of scorpion venom was given by oral on the twenty-eighth day,one time a day,in total 14 days.The spike of external oblique muscle of abdomen（EOMA）were examined to assess the visceral sensitivity of rats;The field potential LTP was observed in control and model young rats by the recording of field potential in hippocampal CA1 region in vitro.RESULTS：The spike amplitude of EOMA was significantly stronger in model young rats than those in control young rats,the spike amplitudes of EOMA were decreased in model rats by oral colon-targeted ball of scorpion venom（P0.05）.The spike amplitudes of LTP was significantly stronger in model young rats than those in control young rats（P0.05）.Compared with model young rats,the spike amplitudes of LTP were significantly decreased by oral colon-targeted ball of scorpion venom（P0.05）.However,there was no significant effect of colon-targeted ball of scorpion venom on control young rats（P0.05）.CONCLUSION： Chronic visceral hypersensitivity could be inhibited by Oral colon-targeted ball of scorpion venom in model young rats.