双酚 A对成年 C57BL/6J小鼠糖、脂代谢的影响

目的：研究双酚A（BPA）长期暴露对C57BL/6J小鼠血糖、血脂的影响。方法：8周龄雄性小鼠随机分为正常对照组、溶剂对照组、低剂量BPA组、高剂量BPA组,分别给予蒸馏水、玉米油、0.5mg·kg^-1·d^-1和50mg·kg^-1·d^-1BPA,90d后,进行口服糖耐量（OGTT）测定,血TC、TG、HDL-C、LDL-C含量测定,肝脏病理切片及肝脏脂肪酸合成酶（FAS）、乙酰辅酶A羧化酶（ACC）、硬脂酰CoA脱饱和酶1（SCD1）和葡萄糖激酶（GCK）表达水平的测定。结果：与正常对照组和溶剂对照组相比,低剂量BPA组小鼠糖耐量存在明显异常（P〈0.05）;血脂各指标出现异常,血TC、TG、LDL-C升高,HDL-C下降（P均〈0.05）;高剂量BPA组TC、TG和HDL-C水平差异无统计学意义（P均〉0.05）,而LDL-C水平升高（P〈0.05）。低剂量BPA组肝脏FAS、ACC、SCD1、GCK蛋白表达升高,肝脏出现明显的脂质沉积;而高剂量BPA组仅ACC和GCK蛋白水平表达略有升高,其余蛋白无明显变化。结论：长期低剂量BPA接触会引起机体糖、脂代谢紊乱。

Effects of Bisphenol A on metabolismof glucose and lipid in mice

AIM：To investigate the effects of Bisphenol A（BPA）on lipid metabolism and glucose metabolism in mice.METHODS：The 8-week-old male C57BL/6Jmice were divided randomly into four groups：blank group,solvent group,low-and high-dosage group.The blank group and solvent group were administrated with distilled water and corn oil respectively by gavage,and other two groups were administered with BPA at the dosages of 0.5mg·kg^-1·d^-1nd 50mg·kg^-1·d^-1espectively with the same route.90days later,oral glucose tolerance test（OGTT）was carried out in all the mice.Contents of serum total triglycerides（TG）,total cholesterol（TC）,high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）were detected.At the same time,the hepatic steatosis situation and protein expression levels of FAS,ACC,SCD1,GCK in liver were tested.RESULTS：Compared with the blank group and solvent group,the glucose tolerance in the low-dosage group was abnormal（P〈 0.05）,the levels of TC,TG,LDL-C were increased while the level of HDL-C was reduced（P〈0.05）.Compared with the blank group and solvent group,the levels of TC,TG and HDL-C in the high-dosage group was similar,there were no obviously difference（P〉0.05）,the level of LDL-C in the high-dosage group was increased（P〈0.05）.Protein expression level of FAS,ACC,SCD1and GCK in liver were higher in lowdosage proup and there was lipid depositions in liver,while ACC and GCK were higher in high dosage group.CONCLUSION：The exposure to low-dose BPA for a long time may cause glucose and lipid metabolism disorders.