丹参与电针对脑缺血再灌注损伤大鼠 GFAP和iNOS表达的影响

目的：观察脑缺血再灌注损伤后大鼠脑胶质原纤维酸性蛋白（GFAP）及诱导型一氧化氮合酶（iNOs）的表达，以及丹参与电针对其表达的影响。方法：将60只SD犬鼠随机分为假手术组、模型组、丹参组、电针组、丹参＋电针组，采用大脑中动脉线栓法建立脑缺血再灌注模型。造模后，丹参组给予丹参注射液5g／kg腹腔注射，电针组刺激“百会”、“大椎”穴，疏密波，频率2～15HZ，持续30min，丹参＋电针组给予丹参注射液腹腔注射和电针，各组治疗均每天1次，连续4d。进行神经功能缺损评分、干湿重法测脑组织含水量，采用HE染色观察脑组织的病理形态学变化，免疫组织化学染色法检测各组大鼠纹状体GFAP、iNOS的表达。结果：模型组神经功能缺损评分和脑组织含水量均明显高于假手术组（P〈0．01）；与模型组比较，丹参组、电针组、丹参＋电针组神经功能缺损评分和脑组织含水量均显著降低（P〈O．05或P〈0．01），并且丹参＋电针组的评分和含水量显著低于丹参组、电针组（P〈O．05或P〈0．01）；HE染色显示的病理形态学改变与上述一致。模型组GFAP、iNOS的表达均明显高于假手术组（P〈0．01）；与模型组比较，丹参组、电针组、丹参＋电针组GFAP、iNOS的表达均明显降低（P〈0．05或P〈0．01），并且丹参＋电针组的表达明显低于丹参组、电针组（P〈0．05或P〈0．01）。结论：丹参和电针对脑缺血再灌注损伤有神经保护作用，可能与其抑制星型胶质细胞的过度活化，降低iNOS的表达有关，且二者合用效果更佳。

Effects of salvia miltiorrhiza and electroacupuncture on the expres sions of glial fibrilliary acidic protein and iNOS in rats with cerebral ischemia-reperfusion injury

AIM. To observe the effects of salvia miltiorrhiza （SM） and electroacupuncture （EA） on the expressions of glial fibrilliary acidic protein （GFAP） and iNOS in rats after focal cer ebral ischemia-reperfusion. METHODS.- 60 Sprague-Dawley rats were randomized into the sham opration, model, SM, EA, and SM＋EA groups. The models rats received intraluminal middle cerebral artery occlusion（MCAO） with a nylon monofilament suture. SM injection at 5 g/ kg were injected into abdominal cavity in the SM group, EA（2-15Hz, dilatational wave） at Baihui and Dazhui was performed to rats in the EA group, lasting for 30 min, SMq-EA group was treated with both SM abdominal cavity injection and electroacupuncture therapy, once a day for 4 days. To observe the neurologic impairment score in each group, and the water content of brain tissue was determined by weighing. HE staining was performed to display the pathologi cal changes of the brain tissues, and the expres- sions of GFAP and iNOS in striatum were detec ted by immunohistochemical method. RESULTS. The neurologic impairment score and water con tent of brain tissue in model group were signifi cantly increased compared with sham operation group（P~0.01）. Compared with model group, the neurologic impairment score and water con- tent of brain tissue in SM group, EA group andSMq-EA group were significantly decreased （P d0.05,P~0.01）, and the score and water con- tent in SM~EA group was obviously lower than those of the SM group and the EA group（P% 0.05, P%0.01）. The pathological changes of the brain tissues of four group rats were consist- ent with the above result. The expression of GFAP and iNOS in model group was obviously increased compared with sham operation group （P%0.01）. Compared with model group, the expression of GFAP and iNOS in SM group, EA group and SMq-EA group were significantly de- creased （P%0.05, P%0.01）. The expression of GFAP and iNOS in SMq-EA group was obvi ously lower than those of the SM group and the EA group （P 〈 0.05, P 〈 0.01 ）. CONCLU SION： SM and EA has a neuroprotective activity in cerebral ischemia-reperfusion neuronal injury, which is probably related to inhibit the excessive activation of astrocytes, decrease the expression of iNOS, besides, the effects of SM combined with EA was higher than the SM group or the EA group.