水合氯醛诱发 RAW264.7巨噬细胞凋亡及其机制

目的：探讨水合氯醛处理对RAW264．7巨噬细胞凋亡的影响及其机制。方法：以不同浓度的水合氯醛对RAW264．7巨噬细胞处理不同时间，采用形态学观察、AnnexinV－FITC／PI双染细胞凋亡检测试剂、Hochest33258染色和DNA1adder试剂检测RAW264．7巨噬细胞的凋亡情况，并检测Fas／FasL表达情况。结果：水合氯醛处理后RAW264．7巨噬细胞形态由梭形变圆直至脱落悬浮；AnnexinVFITC／PI双染检测显示水合氯醛处理可诱导RAW264．7巨噬细胞从早期向晚期凋亡，Hochest33258染色和DNA1ad－der检测均显示诱导凋亡；同时高表达Fas，但不表达FasL。结论：水合氯醛可通过Fas／FasL途径诱导RAW264．7巨噬细胞细胞凋亡。

Apoptosis of RAW264.7 macrophages induced by chloral hydrate and its mechanisms

To investigate the effect of chloral hydrate treatment on the apoptosis of RAW264.7 macrophages and the mechanisms of apoptosis. METHODS： To treat RAW264. 7 maerophages by chloral hydrate with different concentrations and different times, then to ob- serve the morphological changes. Also, Annexin V-FITC/PI double staining assay, Hochest 33258 and and DNA ladder assay were used to detect the apoptosis, and the expression of Fas/ FasL. RESULTS： After treatment with chloral hydrate, the morphology of RAW264.7 macro- phages changed from fusiform to round, and suspension at last. Annexin V-FITC/PI doublestaining assay showed RAW264.7 macrophages were from early to late apoptosis after chloral hydrate treatment. Hochest 33258 and and DNA ladder assay showed chloral hydrate treatment can induced the apoptosis of RAW264.7 macro phages. Also, the apoptotic RAW264.7 macro phages expressed higher levels of Fas, but did not express FasL. CONCLUSION： Chloral hy drate treatment could induce RAW264.7 macro phages apoptosis via the Fas/FasL pathway.