奥美拉唑、铝碳酸镁和米索前列醇对大鼠急性胃粘膜损伤保护作用及机制

目的：比较奥美拉唑（omeprazole，Ome）、铝碳酸镁（Talcid，Tal）和米索前列醇（misoprostol，Mis）对急性胃粘膜损伤的保护作用及机制。方法：复制大鼠水浸-束缚应激（WRS）性渍疡模型，计算粘膜损伤指数（UI）；TUNEL法检测细胞凋亡、免疫组化法检测诱导型一氧化氮合酶（iNOS）表达，原位杂交法检测细胞间隙连接蛋白43（CX43）表达。结果：大鼠分别灌服Ome、Mis和Tal后，均能明显减轻胃粘膜损伤，降低胃粘膜细胞凋亡发生率，抑制iNOS表达，增加CX43表达（P〈0．01），其中Ome、Mis降低胃粘膜细胞凋亡发生优于Tal，而Mis和Tal增加CX43表达强于Ome。结论：三种药物作用于急性胃粘膜损伤发生的不同环节，明显抑制细胞凋亡发生，抑制iNOS表达，促进CX43表达，对应激性溃疡（SU）有明显的防治作用。

Protective effect of omeprazole,Talcid and misoprostol on acute gastric mucosa lesion

AIM: To investigate the protective effect of antiulcer agents omeprazole,misoprostol and Talcid on the acute gastric mucosa lesion.METHODS: Water immersion-restraint stress model were performed with SD rats.Mucosa lesion was evaluated by ulcer index.Apoptosis cells in gastric mucosa were measured by terminal deoxynucleatidyl transferase mediared dUTP nick and labelling(TUNEL) techniques,while expression of inducible NO synthase(iNOS) was detected by immunohistochemical staining and CX43 by In situ hyberdization. RESULTS: Compared with control group,the groups of pretreatment with Omeprazole,Misoprostol and Talcid showed a significant reduction of damaged mucosa and epithelial cell apoptosis,the effects of Omeprazole and Misoprostol were better than Talcid.Three antiulcer agents decreased iNOS expression and increased CX43 expression,the effect of Misoprostol and Talcid was better than Omeprazole.CONCLUSION: Omeprazole,misoprostol and Talcid can exert the protective effect to counter the acute gastric mucosa lesion with different mechanism.