复方海蛇胶囊对帕金森病小鼠的作用

目的：研究复方海蛇胶囊对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的帕金森病小鼠的作用。方法：采用C57BL/6J小鼠腹腔注射MPTP制备帕金森病动物模型,给药14 d后观察各组小鼠行为活动变化,利用高效液相-电化学检测器检测纹状体内多巴胺（DA）及其代谢产物二羟基苯乙酸（DOPAC）和高香草酸（HVA）等的含量变化。结果：复方海蛇胶囊可以显著对抗MPTP对黑质及纹状体的损伤作用,明显改善帕金森病的症状;与模型组相比,复方海蛇胶囊治疗后可以显著提高小鼠纹状体内DA、DOPAC和HVA的含量（P〈0.01）。结论：复方海蛇胶囊通过保护多巴胺能神经元,提高纹状体内DA的含量,改善帕金森病的症状。

Effects of Sea Cucumber Capsule on the Parkinson's mouse induced by 1-methyl-4-phenyi-1,2,3,6-tetrahydropyridine

AIM：To investigate the effect of Sea Cucumber Capsule on the Parkinson＇s mouse induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）. METHODS：C57BL/6J mice were treated intraperitoneally with MPTP to prepare the PD model.The mice were treated with different doses Sea Cucumber Capsule for 14 days.The behavior tests of mice were observed and the contents of DA and its metabolites in the striatum of each group mice were detected by high performance liquid chromatography with electrochemical detection（HPLC-ECD）.RESULTS：Sea Cucumber Capsule obviously enhanced the model mice＇s capability of motor coordination and prevented the destructive effect of MPTP.Sea Cucumber Capsule was effective against MPTP-induced neurodegeneration of the nigrostriatal dopaminergic neural pathway,and increasing DA and its metabolites＇ levels in the striatum.CONCLUSION：This study proves that Sea Cucumber Capsule could ameliorate the typical symptom of PD by protecting dopaminergic system.