HPLC法测定碘化钾滴眼液中羟苯乙酯的含量

目的建立高效液相色谱法测定碘化钾滴眼液中羟苯乙酯的含量。方法色谱柱为Agilent Eclipse Plus C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.005 mol·L^-1醋酸铵溶液含三乙胺1%,用冰醋酸调节pH值至(5.0±0.5)]-乙腈(50∶50,v/v),检测波长为256 nm,流速为1.0 mL·min^-1,柱温为30℃。结果羟苯乙酯在6.010 0~21.034 9μg·mL^-1浓度范围内呈良好线性关系,r=0.999 9,平均回收率为99.83%,RSD=0.70%(n=9),该色谱方法重现性好,对照品溶液和供试品溶液在室温放置24 h基本稳定。结论该方法简单、快速、准确,可用于测定碘化钾滴眼液中羟苯乙酯的含量。

Determination of Ethylparaben in Potassium Iodide Eye Drops by HPLC

OBJECTIVE To establish an HPLC method for the determination of ethylparaben in potassium iodide eye drops.METHODS The analytic column was Agilent Eclipse Plus C18 (4.6 mm×250 mm,5 μm) and the mobile phase was 0.005 mol·L^-1 ammonium acetate (1 000 mL consisted of 10 mL triethanolamine,p H adjust to 5.0 with acetic)-acetonitrile (50 ∶50,v/v) at a flow rate of 1.0 mL·min^-1. The detection wavelength was 256 nm and the column temperature was 30 ℃ . RESULTS The linear range of ethylparaben was 6.010 0-21.034 9 μg·mL^-1,r= 0.999 9.The average recovery rate of ethylparaben was 99.83% with RSD= 0.70% (n= 9).The method had high reproducibility. The reference solution and testing solution were stable for 24 h in room. CONCLUSION The detection method is simple,quick and accurate,which is suitable for the determination of ethylparaben in potassium iodide eye drops.