HPLC法测定不同来源地黄中梓醇的含量

目的建立地黄中有效成分梓醇的HPLC测定方法,测定不同来源地黄药材中的梓醇含量。方法采用高效液相色谱法,选用C18柱,流动相为甲醇-0.1%磷酸溶液(1∶99),流速1.0 ml.min-1,检测波长为210 nm,柱温为35℃。结果梓醇在25～250 mg.L-1范围内具有良好的线性关系(r=0.999 6),平均加样回收率95.66%,RSD为1.52%(n=9)。结论不同来源地黄中有效成分梓醇的含量差异较大,生地黄中的梓醇含量高于熟地黄中梓醇含量。

Determination of catalpol in radix rehmanniae from different sources by HPLC

Objective To determine the content of catalpol in radix rehmanniae from different sources. Methods Catalpol in radix rehmanniae was determinated by high performance liquid chromatography. The mobile phase consisted of methyl alcohol-0.1% phosphori- cacid （1：99） with the flow rate of 1.0 ml · min-l. The detection wavelength was at 210 nm and column temperature was 35 °C. Results Good linearities were obtained over the range between 25 and 250 mg · L-1 for catalpol. The average recovery of glycyrrhizic acid was 95.66% （ RSD = 1.52%, n = 9）. Conclusion Content of catalpol is variant in different sources. The content of catalpol in radix rehmanniae is higher than that of radix rehmanniae preparata.