| HPLC法测定不同产地博落回中白屈菜红碱和血根碱含量 |
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| 引用本文： | 王曙光,滑培玲,王涵磊,胡惠平.HPLC法测定不同产地博落回中白屈菜红碱和血根碱含量[J].安徽医药,2011,15(4):434-436. |
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| 作者姓名： | 王曙光 滑培玲 王涵磊 胡惠平 |
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| 作者单位： | 安徽中医学院,安徽,合肥,230031;安徽省肺科医院,安徽,合肥,230038;安徽省医学科学研究院,安徽,合肥,230061 |
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| 摘 要： | 目的建立不同产地博落回根中白屈菜红碱和血根碱的HPLC法含量测定方法。方法采用HPLC法,色谱柱:SHI-MADZU C18(4.6 mm×250 mm,5μm),流动相:0.4%磷酸-乙腈(73:27),流速1.2 ml·min-1,柱温30℃。结果白屈菜红碱、血根碱的回收率分别为98.26%,97.73%;RSD分别为1.87%,1.81%。结论利用该法测定博落回总碱中的白屈菜红碱和血根碱,结果准确度高、精密度好,可作为博落回总碱的质量控制方法。
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| 关 键 词： | 不同产地博落回 白屈菜红碱 血根碱 高效液相色谱 |
Determination of chelerythrine and sanguinnarine in macleaya cordata from the different areas by HPLC |
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| WANG Shu-guang,HUA Pei-ling,WANG Han-lei,HU Hui-ping.Determination of chelerythrine and sanguinnarine in macleaya cordata from the different areas by HPLC[J].Anhui Medical and Pharmaceutical Journal,2011,15(4):434-436. |
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| Authors: | WANG Shu-guang HUA Pei-ling WANG Han-lei HU Hui-ping |
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| Institution: | WANG Shu-guang1,HUA Pei-ling1,WANG Han-lei2,HU Hui-ping3(1.Anhui College of Traditional Chinese Medicine,Hefei,Anhui 230031,2.Anhui Lung Hospital,Anhui 230038,3.Anhui Institute of Medical Sciences,Anhui 230061) |
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| Abstract: | Aim To develop an HPLC method for the determination of chelerythrine and sanguinairne in macleaya cordata from different place of production.Methods HPLC was adopted,SHIMADZU C18 column（4.6 mm×250 mm,5 μm）with a mobile phase acetonitrile-0.4%（V/V）phosphoric acid solution（27∶73）by diode array detector at 270 nm.Results The average recoveries of chelerythrine and sanguinarinc were 98.26%,97.73%,respectively.RSD were 1.87%,1.81%respectively.Conclusion The method is sensitive and reliable and can be used as a quality control method of the chelerythrine and sanguinairne in total alkaloid of macleaya cordata. |
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| Keywords: | macleaya cordata in different areas chelerythrine sanguinairne HPLC |
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