高效液相色谱法测定风湿骨痛丸中双酯型生物碱的限度 |
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引用本文: | 刘军玲,班永生,蒲婧哲,张亚中.高效液相色谱法测定风湿骨痛丸中双酯型生物碱的限度[J].安徽医药,2013,17(7):1112-1113. |
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作者姓名: | 刘军玲 班永生 蒲婧哲 张亚中 |
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作者单位: | 安徽省食品药品检验所,安徽,合肥,230051;安徽省食品药品检验所,安徽,合肥,230051;安徽省食品药品检验所,安徽,合肥,230051;安徽省食品药品检验所,安徽,合肥,230051 |
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摘 要: | 目的建立高效液相色谱法测定风湿骨痛丸中双酯型生物碱的限度。方法供试品采用超声处理法,双酯型生物碱中三种成分通过菲罗门C18被检测,柱温30℃,以乙腈—四氢呋喃—0.1 mol.L-1的乙酸铵溶液(每1 000 mL中加冰醋酸0.5 mL)(12.5∶7.5∶80)为流动相,检测波长230 nm。结果乌头碱、次乌头碱、新乌头碱峰分离度均良好,且无干扰。结论此法准确,重复性好,可用于控制该处方中制川乌、制草乌中双酯型生物碱的限度。
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关 键 词: | 双酯型生物碱 乌头碱 次乌头碱 新乌头碱 高效液相色谱法 |
Quantification of three aconitine-type diester alkaloidsin Fengshigutong pills by HPLC |
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Institution: | LIU Jun-ling,BAN Yong-sheng,PU Jing-zhe,et al(Anhui Institute for Drug Control,Hefei,Anhui 230051,China) |
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Abstract: | Objective To establish an HPLC method for determing three alkaloids(aconitine,hypaconitine and mesaconitine) in Fengshigutong pills.Methods The separation was performed on a Phenomenex C18 column(5 μm,4.6 mm × 250 mm) with column temperature of 30℃.The mobile phase consisted of acetonitrile-tetrahydrofuran-0.1 mol.L-1ammonium acetate(per 1 000 mL contains 0.5 mL)(12.5∶ 7.5∶ 80) at the flow rate of 1.0 mL.min-1and detection wavelength at 230 nm.Results The separation showed a good result.Conclusion The method is accurate and reproducible.It can be used for the quality control of Fengshigutong pills. |
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Keywords: | diester alkaloids aconitine hypaconitine mesaconitine HPLC |
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