茶黄素通过上调环状 RNA叉头框蛋白 3表达调控结肠癌细胞增殖及凋亡

目的探讨茶黄素( TF)对结肠癌细胞增殖、凋亡的影响及其对环状 RNA叉头框蛋白 3(circ-Foxo3)的调控作用。方该研究于 2018年 10月至 2019年 12月进行，体外培养人结肠癌细胞 HCT-8，分别使用不同剂量的 TF处理细胞，分别将空载法体( pcDNA)、过表达 circ-Foxo3(pcDNA-circ-Foxo3)转染至 HCT-8细胞，分别将抑制物( si-NC)、抑制 circ-Foxo3(si-circ-Foxo3)转染至 HCT-8细胞，随后加入 TF处理细胞；采用噻唑蓝( MTT)法检测细胞增殖；流式细胞术检测细胞凋亡率；采用实时荧光定量 PCR(qRT-PCR)法检测 circ-Foxo3的表达量。结果 TF可明显降低细胞存活率［(100.00±0.12)%比( 82.93±5.51)%、

Theaflavin regulates colon cancer cell proliferation and apoptosis by up-regulating circ-Foxo3 expression

Objective To explore the effect of theaflavin on the proliferation and apoptosis of colon cancer cells and its regulation oncircular RNA forkhead box protein 3 (circ-Foxo3).Methods This study started in October 2018 and December 2019, human colon can-cer cells HCT-8 were cultured in vitro, cells were treated with different doses of theaflavins, respectively. and empty vector (pcDNA) andoverexpression of circ-Foxo3 (pcDNA-circ-Foxo3) were transfected into HCT-8 cells, respectively. The inhibitor (si-NC) and inhibition of circ-Foxo3 (si-circ-Foxo3) were transfected into HCT-8 cells respectively, and then theaflavin was added to treat the cells. Thiazolyl Blue(MTT) method was used to detect cell proliferation. Flow cytometry was used to detect the apoptosis rate. The expression of circ-Foxo3 was detected by quantitative Real-Time PCR (qRT-PCR) method.Results Theaflavins significantly reduced the cell survival rate (100.00± 0.12)% vs. (82.93±5.51)%, (56.67±12.36)%, (32.26±6.10)%, P<0.05], increased the apoptosis rate (5.96±0.58)% vs. (13.67±1.67)%, (17.23±0.52)%, (21.49±2.00)%, P<0.05], promoted the expression of circ-Foxo3 (1.01±0.01) vs. (1.35±0.10), (1.76±0.07), (2.62±0.39), P<0.05]. Transfection of pcDNA-circ-Foxo3 could significantly reduce cell survival rate (P<0.05), increase apoptosis rate (P<0.05). Transfection of si-circ-Foxo3 could significantly reduce the effect of theaflavins on the proliferation and apoptosis of HCT-8 cells.Conclu. sion Theaflavin inhibits the proliferation of colon cancer cells and promotes apoptosis by up-regulating circ-Foxo3 expression.