紫草素对子宫内膜异位症大鼠异位病灶的影响

目的探究紫草素对子宫内膜异位症(EMs)大鼠异位病灶及子宫内膜组织基质细胞衍生因子-1(SDF-1)、基质细胞衍生因子受体-4(CXCR4)表达的影响。方法雌性SD大鼠120只,随机分为对照组、模型组、实验组及阳性对照组,每组30只。除对照组外,其余3组大鼠均采用自体移植法建立EMs大鼠模型。造模成功后,实验组大鼠灌胃紫草素(10 mg·kg-1·d-1),阳性对照组大鼠灌胃孕三烯酮(0.23 g·kg-1·d-1),对照组与模型组灌胃等量生理盐水,连续干预15 d。干预结束后,测定异位病灶体积;采用苏木精-伊红(HE)染色观察大鼠子宫内膜组织形态学变化;采用酶联免疫吸附(ELISA)法检测血清卵泡刺激素(FSH)、黄体生成激素(LH)、雌激素(E2)、孕激素(P)水平;采用免疫组织化学法检测子宫内膜组织SDF-1、CXCR4蛋白表达。结果对照组、模型组、实验组及阳性对照组大鼠异位病灶体积分别为0,(126.62±79.34),(28.36±24.12),(16.38±14.51)mm3;子宫内膜组织SDF-1蛋白阳性细胞百分比分别为(19.23±2.86)%,(84.38±3.11)%,(62.42±3.73)%,(41.12±4.25)%;CXCR4蛋白阳性细胞百分比分别为(28.06±2.54)%,(78.68±3.23)%,(56.45±2.93)%,(43.13±3.26)%。与模型组比较,实验组大鼠异位病灶的体积缩小,且阳性对照组小于实验组;模型组大鼠血清FSH、LH、P水平较对照组显著降低,与模型组比较,实验组升高,且阳性对照组高于实验组;血清E2水平及子宫内膜组织SDF-1、CXCR4蛋白阳性细胞百分比均升高,与模型组比较,实验组降低,且阳性对照组低于实验组(均P<0.05)。结论紫草素可抑制EMs大鼠异位病灶的发展,同时抑制E2的异常分泌,提高FSH、LH、P水平,降低子宫内膜组织中SDF-1、CXCR4蛋白表达,进而抑制异位内膜的生长,达到治疗EMs的目的。

Effects of shikonin on ectopic lesions of rats with endometriosis

Objective To investigate the effects of shikonin on the expression of striated stromal cell-derived factor-1(SDF-1) and stromal cell-derived factor receptor-4(CXCR4) in endometrial tissue and ectopic lesions of rats with endometriosis(EMs).Methods A total of 120 female Sprague-Dawley rats were randomly divided into control group(n=30), model group(n=30), test group(n=30) and active control group(n=30).Except the control group, the other three groups of rats were established with EMs rat model by autologous transplantation.After successful modeling, rats in the test group were intragastrically administered with shikonin(10 mg·kg-1·d-1), and rats in the active control group were given gestrinone(0.23 g·kg-1·d-1), the control group and the model group were given the same amount of normal saline for 15 d.After the intervention,the volume of ectopic lesions was measured;Morphological changes of endometrium in rats were observed by hematoxylin-eosin(HE) staining;Serum follicle stimulating hormone(FSH),luteinizing hormone(LH),estrogen(E2) and progesterone(P) levels were detected by enzyme-linked immunosorbent assay(ELISA);The expression of SDF-1 and CXCR4 protein in endometrium were detected by immunohistochemistry method.Results The volume of ectopic lesions in the control group,the model group,the test group and the active control group were 0,(126.62 ± 79.34),(28.36 ± 24.12),(16.38 ± 14.51) mm3;The percentage of SDF-1 protein positive cells in endometrial tissues were(19.23 ± 2.86) %,(84.38 ± 3.11) %,(62.42 ± 3.73) %,and(41.12 ± 4.25) %,respectively;The percentage of CXCR4 protein positive cells were(28.06 ± 2.54) %,(78.68 ± 3.23) %,(56.45 ± 2.93) % and(43.13 ± 3.26) %,respectively.Compared with the model group,the volume of ectopic lesions in the test group reduced,and the active control group was smaller than the test group;Compared with the control group,the levels of serum FSH,LH and P in the model group significantly reduced,and the test group was higher than the model group,and the active control group was higher than the test group;The serum E2 level and the percentage of SDF-1 and CXCR4 protein positive cells in endometrial tissues increased,and the test group was lower than the model group,and the active control group was lower than the test group(all P < 0.05).Conclusion Shikonin can inhibit the development of ectopic lesions in EMs rats,inhibit the abnormal secretion of E2,increase the levels of FSH,LH and P,and decrease the expression of SDF-1 and CXCR4 in endometrial tissues,and the growth of the ectopic endometrium is inhibited,and the purpose of treating EMs is achieved.