黄芪提取物对全脑缺血损伤的保护作用

目的　研究黄芪提取物 (EA )对大鼠和小鼠全脑缺血的保护作用。方法　采用小鼠双侧颈总动脉结扎法 ,造成全脑急性缺血模型 ,观察小鼠 12h内存活时间 ,并记录 2、6、12h小鼠死亡率。参照Pulsinelli法制作大鼠全脑缺血再灌注模型 ,观察EA的神经保护作用 ,记录脑电图 (EEG) ,观察并记录大鼠全脑缺血后翻正反射 (rightingreflex ,RR )恢复时间 ,测定脑组织中谷胱甘肽过氧化物酶 (GSH Px)、乳酸脱氢酶 (LDH)、一氧化氮合酶 (NOS)活性 ,测定组织中诱导型NOS(iNOS)的表达并运用图像分析系统定量分析海马区平均光密度值 (meanopticaldensity ,MOD)。 结果　EA能降低急性脑缺血小鼠的死亡率 ,延长 12h内小鼠存活时间。EA能促进大鼠EEG和翻正反射的恢复 ,可显著提高全脑缺血再灌注大鼠脑组织中GSH Px、LDH活性 ,降低NOS活性 ,抑制海马iNOS的表达 ,降低其MOD值。结论　黄芪提取物对大鼠和小鼠全脑缺血损伤具有保护作用

Protective effect of EA against cerebral ischemia-reperfusion injury

AIMTo study the protective effects of EA against cer ebral ischemia-reperfusion injury. METHODSAcute cerebral ischemia w as produced by the occlusion of bilateral common carotid arteries of mice .The s urvival time in 12 h of the mice was observed, and the mortalities in 2 h, 6 h, 12 h were recorded. Pulsinelli four-vessel occlusion method was used to make ce rebral ischemia-reperfusion model of rats. The EEG and the reappearing time of righting reflex were recorded and the activity of GSH-Px,LDH,NOS in the brain w as tested. Researching the iNOS expression in hippocampiwith immunocytochemistry method and measure the mean optical density. RESULTSEA can decrease the mortalities and prolong the survival t ime in 12 h of the mice. EA can promote the recovery of the EEG and the RR after cerebral ischemia-reperfusion operation in rats and enhance the activities of the GSH-Px LDH, reduce the activity of the NOS. EA also inhibits the expression of iNOS and reduce it's value of mean optical density in hippocampi of the rat s. CONCLUSIONEA has protective effects against cerebral ischemai-r eperfusion injury.