| 肾上腺髓质素基因敲除小鼠单侧输尿管结扎肾脏α-SMA表达特点及缬沙坦的作用 |
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| 引用本文： | 许庆友,丁英钧,朱立,刘力剑,刘丽,赵玉庸,下泽达雄,藤田敏郎.肾上腺髓质素基因敲除小鼠单侧输尿管结扎肾脏α-SMA表达特点及缬沙坦的作用[J].中国药理学通报,2006,22(7):819-823. |
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| 作者姓名： | 许庆友 丁英钧 朱立 刘力剑 刘丽 赵玉庸 下泽达雄 藤田敏郎 |
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| 作者单位： | 1. 河北医科大学中西医结合学院,河北,石家庄,050091
2. 东京大学肾脏内分泌内科,日本,东京 |
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| 基金项目： | 国家留学基金委留学基金 |
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| 摘 要： | 目的观察肾上腺髓质素基因缺失状态下小鼠肾脏α-SMA的表达特点及缬沙坦的作用。方法结扎单侧输尿管方法制备肾间质纤维化实验动物模型,采用免疫组化、RT-PCR方法检测α-SMA的定位与mRNA表达。结果单侧输尿管结扎小鼠肾脏α-SMA表达明显高于假手术组(P<0.01),其中肾上腺髓质素基因敲除小鼠高于野生小鼠,缬沙坦可下调其表达。结论单侧输尿管结扎可诱导肾小管上皮细胞表型转化,肾上腺髓质素基因缺失状态下其表达增强,血管紧张素受体拮抗剂缬沙坦可部分抑制其转化。
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| 关 键 词： | α-平滑肌肌动蛋白 肾上腺髓质素 单侧输尿管结扎 缬沙坦 基因敲除 |
| 文章编号： | 1001-1978(2006)07-0819-05 |
| 收稿时间： | 02 8 2006 12:00AM |
| 修稿时间： | 04 6 2006 12:00AM |
Expression of α-smooth muscle actin in adrnomedullin knockout mice with unilateral ureteral obstruction and treated by valsartan |
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| XU Qing-you,DING Ying-jun,ZHU Li,LIU Li-jian,LIU Li,ZHAO Yu-yong,SHIMOSAWA Tatsuo,FUJITA Toshiro.Expression of α-smooth muscle actin in adrnomedullin knockout mice with unilateral ureteral obstruction and treated by valsartan[J].Chinese Pharmacological Bulletin,2006,22(7):819-823. |
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| Authors: | XU Qing-you DING Ying-jun ZHU Li LIU Li-jian LIU Li ZHAO Yu-yong SHIMOSAWA Tatsuo FUJITA Toshiro |
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| Institution: | 1. College of Integrated Traditional and Western Medicine, Hebei Medical University, Shijiazhuang 050091, China ;2. Dept of Nephrology and Endocrinology, Tokoy University, Tokoy, Japan |
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| Abstract: | Aim To observe the expression ofα-smoothmuscle actin(α-SMA) in adrenomedullin knockoutmice with unilateral ureteral obstruction(UUO) andthe effectofvalsartan.Methods Renal interstitial fi-brosis animals were made with UUO、α-SMA and theexpression of mRNA were examined in adrnomedullinknockout(AMKO) mice and wild type(WT) mice byimmunohistochemistry and RT-PCR.Results The ex-pression ofα-SMA in UUO groups were up-regulatedsignificantly compared with sham group(P<0.01),specically in AMKO group.Valsartan has the effect indown-regulating its over-expression.Conclusion Tu-bular epithelial-myofibroblast transdifferentiation wasinduced by UUO and over-expression in AMKO mice.Valsatan has a role to inhibit it partly. |
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| Keywords: | α-smooth muscle actin adrenomedullin unilateral ureteral obstruction valsartan gene knock-out |
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