| 女贞子多糖对黑色素瘤细胞黏附能力的影响 |
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| 引用本文： | 李璘,邱蓉丽,程革,谢付波,李寰舟,陆茵. 女贞子多糖对黑色素瘤细胞黏附能力的影响[J]. 中国药理学通报, 2009, 25(10) |
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| 作者姓名： | 李璘 邱蓉丽 程革 谢付波 李寰舟 陆茵 |
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| 作者单位： | 南京中医药大学药学院,江苏,南京,210029 |
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| 基金项目： | 江苏省高校自然科学基金资助项目，江苏省中医药管理局基金资助项目 |
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| 摘 要： | 目的探讨女贞子多糖对黑色素瘤细胞B16BL6黏附能力的影响。方法观察女贞子多糖对黑色素瘤细胞黏附力的抑制作用,分别将琼脂糖和Matrigel铺于细胞板中,用女贞子多糖作用后,MTT法观察女贞子多糖对B16BL6黏附能力的影响;同时观察了女贞子多糖对黏附分子E-cadherin表达的影响。结果女贞子多糖抑制了黑色素瘤细胞的粘附能力,抑制黑色素瘤粘附分子E-cadherin的表达。结论女贞子多糖具有抑制黑色素肿瘤细胞的黏附,破坏了肿瘤细胞的整体性,利于女贞子多糖的抗肿瘤作用。
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| 关 键 词： | 女贞子多糖 黑色素瘤细胞 黏附 E-cadherin MTT法 RT-PCR |
The adhesion effect of Ligustrum lucidum Ait polysaccharide on melanoma B16BL6 in vitro |
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| LI Lin,QIU Rong-li,CHENG Ge,XIE Fu-bo,LI Huan-zhou,LU Yin. The adhesion effect of Ligustrum lucidum Ait polysaccharide on melanoma B16BL6 in vitro[J]. Chinese Pharmacological Bulletin, 2009, 25(10) |
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| Authors: | LI Lin QIU Rong-li CHENG Ge XIE Fu-bo LI Huan-zhou LU Yin |
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| Abstract: | Aim To investigate the effects of the polysaccharides from Ligustrum lucidum Ait(LLP)on melanoma B16BL6 adhesion in vitro.Methods The effect of LLP on melanoma B16BL6 adhesion in vitro was evaluated by MTT assay on agarose and matrigel.Also the effect of LLP on the expression of adhesion molecule E-cadhesion in melanoma B16BL6 in vitro was studied.Results LLP had an obvious inhibition on melanoma B16BL6 adhesion and decreased the expression of adhesion molecule E-cadhesion in melanoma B16BL6 in vitro.Conclusion LLP can inhibit the adhesion of melanoma B16BL6 cells in vitro and dampen the system of B16BL6 cells,and then be good at the effect of LLP in the of anti-tumor activity. |
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| Keywords: | E-cadherin RT-PCR |
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