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扇贝裙边糖胺聚糖体外抗I型单纯疱疹病毒实验研究
引用本文:于囡,刘赛,王海桃,高艳丽.扇贝裙边糖胺聚糖体外抗I型单纯疱疹病毒实验研究[J].中国药理学通报,2008,24(2).
作者姓名:于囡  刘赛  王海桃  高艳丽
基金项目:国家自然科学基金 , 山东省中医药科技发展基金
摘    要:目的研究扇贝裙边糖胺聚糖(glycosaminoglycan from Scallop Skirt,SS-GAG)体外抗单纯疱疹病毒Ⅰ型(HSV-Ⅰ)的作用。方法运用单纯疱疹病毒Ⅰ型(HSV-ⅠSM44株),并以非洲绿猴肾细胞(Vero细胞)为宿主细胞,通过观察病毒感染后的细胞变性反应(cytopathic effect,CPE)和运用MTT比色法,检测不同浓度药物SS-GAG对Ⅰ型单纯疱疹病毒是否有直接的灭活作用、对HSV-Ⅰ感染复制的抑制活性以及对HSV-Ⅰ感染细胞的综合作用等,并观察药物对Vero细胞的毒性作用。结果与病毒对照组相比,SS-GAG各浓度组(100、50、25mg.L-1)能有效地保护经HSV-Ⅰ感染的Vero细胞,使细胞活性增强(P<0.01);并减弱HSV-Ⅰ导致的病变效应,抑制病毒的复制。此作用随着药物浓度的增加而增强。但是SS-GAG对HSV-Ⅰ没有直接的灭活作用;SS-GAG在50~1600mg.L-1浓度范围内对Vero细胞无明显的细胞毒性。结论SS-GAG在体外实验系统中显示出明显的保护宿主细胞抵抗HSV-Ⅰ病毒感染的活性作用。

关 键 词:扇贝裙边  糖胺聚糖  单纯疱疹病毒Ⅰ型  抗病毒作用

Experimental study of the antiviral effect of glycosaminoglycan from scallop skirt on herpes simplex virus type Ⅰ in vitro
YU Nan,LIU Sai,WANG Hai-tao,GAO Yan-li.Experimental study of the antiviral effect of glycosaminoglycan from scallop skirt on herpes simplex virus type Ⅰ in vitro[J].Chinese Pharmacological Bulletin,2008,24(2).
Authors:YU Nan  LIU Sai  WANG Hai-tao  GAO Yan-li
Abstract:Aim To study the antiviral effects of glycosaminoglycan from Scallop Skirt(SS-GAG)on herpes simplex virus typeⅠ(HSV-Ⅰ)in vitro.Methods The direct effect on virus,the influences of SS-GAG on virus reproduction and synthesis effect in virus infecting cells were investigated in vitro utilizing the herpes simplex virus typeⅠand taking vero cell as host cell by MTT colorimetry and observing cytopathic effect.The cytoxicity of SS-GAG was measured by MTT colorimetry.Results Compared with the virus control group,SS-GAG-treated group could protect the vero cells effectively and heighten the cell activity(P<0.01).SS-GAG-treated group could lessen the cytopathic effect induced by HSV-Ⅰ and inhibit virus replication.These effects became stronger with the increase of concentrations of SS-GAG.But SS-GAG was not observed to have a direct inhibitory effect on the virus.The SS-GAG did not show cell toxicity on vero cells within the concentrations of 50~1600 mg·L-1.Conclusion SS-GAG showed siginificant activity of protecting host cells and anti-viral effect on HSV-Ⅰ in vitro.
Keywords:scallop skirt  glycosaminoglycan  herpes simplex virus typeⅠ(HSV-Ⅰ)  antiviral effect
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