| 黄芪提取物对大鼠局灶性脑缺血再灌注损伤后炎症因子及细胞凋亡的作用 |
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| 引用本文： | 尹艳艳,李卫平,王绍斌,何婷,明亮.黄芪提取物对大鼠局灶性脑缺血再灌注损伤后炎症因子及细胞凋亡的作用[J].中国药理学通报,2005,21(12):1486-1489. |
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| 作者姓名： | 尹艳艳 李卫平 王绍斌 何婷 明亮 |
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| 作者单位： | 安徽医科大学药理学教研室,安徽,合肥,230032 |
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| 基金项目： | 安徽省科研项目，安徽省自然科学基金 |
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| 摘 要： | 目的研究黄芪提取物(EA)对局灶性脑缺血再灌注损伤的保护作用机制。方法采用线栓法大鼠局灶性脑缺血再灌注损伤模型,以免疫组化法观察EA对缺血再灌注后大鼠脑组织中TNFα表达的影响、以放免法观察对IL1β水平的影响、以TUNEL法观察对脑组织细胞凋亡的影响。结果与模型组比较,EA(20、40、80mg·kg-1,ig)能减少TNFα的表达、降低IL1β水平和减少细胞凋亡数。结论EA对大鼠局灶性脑缺血再灌注后脑组织中TNFα及IL1β的升高和神经元的凋亡有明显的抑制作用。
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| 关 键 词： | 黄芪提取物 缺血再灌注 炎症因子 凋亡 |
| 文章编号： | 1001-1978（2005）12-1486-04 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005-08-04 |
Effect of extract of astragalus on inflammatory factor and apoptosis after focal cerebral ischemia/reperfusion injury in rats |
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| YIN Yan-yan,LI Wei-ping,WANG Shao-bin,HE Ting,MING Liang.Effect of extract of astragalus on inflammatory factor and apoptosis after focal cerebral ischemia/reperfusion injury in rats[J].Chinese Pharmacological Bulletin,2005,21(12):1486-1489. |
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| Authors: | YIN Yan-yan LI Wei-ping WANG Shao-bin HE Ting MING Liang |
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| Abstract: | Aim To study the mechanisms of protective effects of extract of astragalus against focal cerebral ischemia/reperfusion injury in rats. Methods Focal cerebral ischemia was induced by intraluminal thread occlusion of middle cerebral artery. Immunohistochemistry was used to determine expression of TNF-α,IL-1β was measured by radioimmunity, and the apoptosis was observed by TUNEL. Results EA(20、40、80 mg·kg -1,ig)could decrease the expression of TNF-α and the level of IL-1β and reduce cell apoptosis. Conclusion EA has inhibitory action on the increase in the expression of TNF-α,the level of IL-1β and the apoptosis of neurons induced by focal cerebral ischemia/reperfusion. |
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| Keywords: | extract of astragalus ischemia/reperfusion inflammatory factor apoptosis |
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