白芍总苷对糖尿病大鼠肾脏保护作用及部分机制

目的探讨白芍总苷(TGP)对糖尿病大鼠肾脏保护作用及部分机制。方法应用链脲佐菌素诱导大鼠糖尿病模型,同时每日灌胃给予TGP(50,100,200mg·kg-1,连续8wk。结果TGP给药呈剂量依赖性降低糖尿病大鼠AER的增加。TGP给药(50mg·kg-1)大鼠肾小球平均容量(VG)明显低于模型组,肾小管-间质损伤指数(TII)较模型组有所下降,但差异无统计学意义;TGP给药(100,200mg·kg-1VG与TII均明显低于模型组。模型组肾组织MDA含量明显高于对照组,TGP给药(200mg·kg-1)大鼠肾组织MDA含量明显低于模型组。Western blot显示模型组肾组织1α(IV)Ⅳ型胶原蛋白表达较对照组增加2.7倍,TGP给药(50、100、200mg·kg-1)8wk分别可使肾组织1α(IV)Ⅳ型胶原蛋白表达下降47.9%、60.4%与72.9%。模型组肾组织ICAM-1及TGFβ1蛋白表达明显高于对照组,TGP给药(50,100,200mg·kg-1)肾组织ICAM-1与TGFβ1蛋白表达明显低于模型组。结论TGP对糖尿病大鼠肾脏保护作用机制部分与其抑制肾组织中ICAM-1及TGFβ1蛋白表达有关。

Effect of total glucosides of paeony on the expression of intercellular adhesion molecule-1 and transforming growth factor 1 protein in the kidney in experimental diabetes

Aim To investigate the effect of total glucosides of paeony (TGP) on the expression of intercellular adhesion molecule-1 (ICMA-1) and transforming growth factor 1 (TGF 1) protein in the kidney in experimental diabetes. Methods Diabetes was induced with streptozotocin in Sprague-Dawley rats. TGP (50, 100, 200 mg·kg-1·d-1) was orally administered once a day for 8 wk to rats. Results Elevated urinary albumin excretion rate (AER) was markedly attenuated by TGP treatment with 50, 100 and 200 mg·kg-1. Increased malondialdehyde (MDA) level was only significantly reduced by TGP treatment with 200 mg·kg-1. Elevated glomerular volume was significantly attenuated by TGP treatment with 50, 100 and 200 mg·kg-1, and increased indices for tubulointerstitial injury were only ameliorated by TGP treatment with 100 and 200 mg·kg-1. Western blot analysis noted that the expression of 1 type Ⅳ collagen protein was increased 2.7 -fold in the kidney in diabetic rats, TGP treatment with 50, 100 and 200 mg·kg-1 could reduced increased expression of 1 type Ⅳ collagen protein by 47.9 %, 60.4 % and 72.9 %. The expression of ICAM-1 and TGF 1 protein in the kidney were significantly increased in diabetic rats, which were all significantly inhibited by TGP treatment. Conclusion Our data suggest that TGP treatment ameliorates early renal injury via the inhibition of oxidative stress and overexpression of ICAM-1 and TGF-1 in renal tissue in diabetic rats.