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小檗碱对人胃癌MGC-803细胞生长抑制及诱导凋亡的作用
引用本文:谭宇蕙,陈冠林,郭淑杰,李燕红,陈蔚文. 小檗碱对人胃癌MGC-803细胞生长抑制及诱导凋亡的作用[J]. 中国药理学通报, 2001, 17(1): 40-43
作者姓名:谭宇蕙  陈冠林  郭淑杰  李燕红  陈蔚文
作者单位:1. 广州中医药大学生化教研室,
2. 广州中医药大学脾胃研究所,
基金项目:广东省自然科学基金!(No 970 2 2 6 ),国家中医药管理局科研基金资助课题!(No 97Y0 31)
摘    要:目的 研究小檗碱对胃癌细胞株生长抑制、诱导凋亡作用 ,及其作用浓度、时间与细胞数目、凋亡程度的关系。方法 苔盼蓝细胞计数 ,MTT染色 ,甲基绿 派诺宁染色观察凋亡特征及计数 ,流式细胞仪技术 ,琼脂糖电泳技术分析药物对DNA作用。结果 小檗碱能抑制胃癌细胞MGC 80 3的生长。 8mg·L-1、4mg·L-1、2mg·L-1、1mg·L-1小檗碱 72h的抑制率分别为 10 0 %、80 4%、5 1 5 %、8 5 %。小檗碱作用细胞后 ,细胞表现出较为典型的细胞凋亡形态 :细胞核固缩 ,染色质凝集断裂 ,颗粒含量增加等 ;72h死亡的细胞中抗拒苔盼蓝染色者 ,仍高达 6 0 %~ 82 % ,胞膜完整 ;流式细胞仪DNA直方图上出现典型亚二倍体峰 ;琼脂糖凝胶电泳分析表明 :小檗碱可使染色质断裂 ,且发生于细胞膜结构被破坏之前 ,DNA形成大片段 ,但不形成小片段。结论 小檗碱体外能抑制胃癌MGC 80 3细胞增殖和诱导细胞凋亡 ,其细胞毒性 (致细胞坏死作用 )较弱

关 键 词:小檗碱  胃肿瘤  生长抑制  细胞凋亡

The effect of berberine in inhibition of proliferation and induction of apoptosis of gastric cancer cell MGC-803
TAN Yu-Hui,CHEN Guan-lin,GUO Shu-Jie,LI Yan-Hong,CHEN Wei-wen. The effect of berberine in inhibition of proliferation and induction of apoptosis of gastric cancer cell MGC-803[J]. Chinese Pharmacological Bulletin, 2001, 17(1): 40-43
Authors:TAN Yu-Hui  CHEN Guan-lin  GUO Shu-Jie  LI Yan-Hong  CHEN Wei-wen
Abstract:AIM To explore the effect of berberine in inhibition of proliferation and induction of apoptosis of gastric cancer cell MGC-803.METHODS The MGC-803 cell morphology and cell counting were studied by Trypan blue, MTT, Methyl green pyronin stain assay, the MGC-803 cell DNA changes were investigated by flow cytometry and agarose gel electrophoresis.RESULTS Berberine could significantly inhibit the growth of MGC-803. After 48 hours of treatment with different concentration berberine (8, 4, 2, 1 mg*L-1), the inhibitive rate of MGC-803 were 86.7%, 65.9%, 20.0%, 0%, respectively. Meanwhile, MGC-803 showed typical apoptosis features: cell shrinkage, nuclear condensation, DNA fragmentation and formation of apoptotic bodies. A typical subdiploid peak before G0/G1 phase was observed by flow cytometry. The Trypan blue stain achromatophilia rates of death cells, which with well membranes, were between 60%~82%. Agarose gel electrophoresis analysis, which showed that the chromosomes of MGC-803 were broken by berberine before the structures of membrane were damaged, while DNA broke into long fragments rather than small fragments, supported that the activation effect of berberine on nuclease was mild.CONCLUSION Berberine could significantly inhibit proliferation of gastric cancer cell MGC-803 and induced apoptosis, and the cytotoxicity of berberine on MGC-803 was weak.
Keywords:berberine  stomach neoplasms  proliferate inhibition  apoptosis
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