| 甲壳胺体外对小鼠巨噬细胞PKC和DAG的作用研究 |
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| 引用本文： | 梁东升,李明春,安丰妹,王月军,袁锦华.甲壳胺体外对小鼠巨噬细胞PKC和DAG的作用研究[J].中国药理学通报,2003,19(6):643-645. |
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| 作者姓名： | 梁东升 李明春 安丰妹 王月军 袁锦华 |
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| 作者单位： | 1. 解放军第401医院药剂科,青岛,266071
2. 解放军第401医院全军手外科中心,青岛,266071 |
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| 基金项目： | 军队“十五”医药卫生科研基金,No 01MB047 |
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| 摘 要： | 目的 观察甲壳胺体外对小鼠腹腔巨噬细胞蛋白激酶C(PKC)活性和二酰基甘油(DAG)合成的影响。方法分别采用反相离子对高效液相色谱法与放射免疫测定技术测定PKC活力和DAG含量。结果 ①甲壳胺剂量依赖性引起巨噬细胞中PKC活性明显增强并使之发生质膜转位,达峰时间为25min,1.5 h恢复到基础水平;②甲壳胺促进巨噬细胞中DAG的合成,达峰时间为30 s,3 min恢复到基础水平。结论 甲壳胺的免疫增强作用与其增强巨噬细胞中PKC活性和DAG合成有关,DAG/PKC信号途径参与了其对巨噬细胞的活化过程。
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| 关 键 词： | 甲壳胺 巨噬细胞 蛋白激酶C 二酰基甘油 信号转导 |
| 文章编号： | 1001-1978(2003)06-0643-03 |
| 修稿时间： | 2002年12月10 |
Effect of chitosan on PKC activity and DAG concentration in murine peritoneal macrophages |
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| LIANG Dong-Sheng,LI Ming-Chun,AN Feng-Mei,WANG Yue-Jun,YUAN Jin-Hua.Effect of chitosan on PKC activity and DAG concentration in murine peritoneal macrophages[J].Chinese Pharmacological Bulletin,2003,19(6):643-645. |
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| Authors: | LIANG Dong-Sheng LI Ming-Chun AN Feng-Mei WANG Yue-Jun YUAN Jin-Hua |
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| Abstract: | ABSTRACT AIM To investigate the effect of ch-itosan on protein kinase C (PKC) activitives and diacyl glycerol(DAG)concentration in murine peritoneal macrophages. METHODS A new ion-pair reversed-phase high liquid chromatography and ra-dioimmunoassay were used to determinate the activity of PKC and the concentration of DAG respectively. RESULTS ( 1) Chitosan induced activation and translocation of PKC in murine peritoneal macrophages. The peak time was 25 min and the activity of PKC came back the basic level at 1. 5 h. (2)Chitosan increased the production of DAG in murine peritoneal macrophages. The peak time was 30 s and the concentration of DAG came back the basic level at 3 min. CONCLUSION The im-munopoteniating effect of chitosan may be associated with the channel of DAG /PKC. |
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| Keywords: | chitosan macrophages protein ki- nase C diacyl glycerol:signal transduction |
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