8-氯腺苷对小鼠肝癌H_(22)细胞的抑瘤作用研究 |
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摘 要: | 8-氯腺苷对小鼠肝癌H22实体瘤的抑瘤率最高可达71.7±13.3%(iP)和66.1±4.46%(iv)(100mg·kg-1d-1×7d)。腹水型H22细胞经8-氯腺苷作用24h后,cAMP含量增加,DAG含量下降;PKAI型调节亚基在药物作用后明显下降;凝胶阻滞分析表明,反式转录因子CREB结合活性增加;原位杂交结果显示P53抗癌基因表达增强。以上结果提示,8-氯腺苷抑瘤作用机制与第二信使系统和基因表达调控过程有关。
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关 键 词: | 8-氯腺苷;抑瘤作用;第二信使系统;抗癌基因 |
Effects of 8-chloroadenosine on murine hepatoma H_(22) cells |
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Abstract: | Chloroadenosine showed marked activity against murine hepatoma H22 solid tumor,at 100 mg·kg-1· d-1×7d. The inhibition rates of H22 were 71.3±13.3%(P<0.01)and 66.1±4.46%(P<0.01) by ip.and iv.administration respectively.8-Cl-Ado increased intercellular cAMP concentration but decreased disylglycerol amount in H22 ascites cells.Photoaffinity labeling/SDS-polyacrylamide gel electrophoresis assay indicated that 8-Cl-Ado greatly decreased R I(regulatory subunit of PKA)in the Cytosol of H22 Cells.Gel retardation analysis demonstrated the enhancement of CRE-binding activity after treatment for 24 hours with 8-Cl-Ado.Using in site hybridization,8-Cl-Ado induced antioncogene p53 expression. |
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Keywords: | chloroadenosine hepatoma signal transduction antioncogene |
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