灵芝多糖肽对ECV304细胞氧化损伤的保护作用

目的研究灵芝多糖肽对ECV304氧化损伤的保护作用。方法培养ECV304细胞,以叔丁基氢过氧化物(tBOOH)为氧化剂损伤细胞,造成氧化损伤模型,培养液中加入不同浓度灵芝多糖(12.5、25、50、100mg.L-1),以MTT法测细胞存活率;以光镜、电镜检测细胞形态学改变及线粒体损伤;用AnnexinV/PI双标记细胞,流式细胞检测细胞凋亡的百分率。结果灵芝多糖(12.5、25、50、100mg.L-1)可减少叔丁基氢过氧化物对ECV304的氧化损伤,MTT检测灵芝多糖给药组,ECV304细胞存活率增加。光镜下可见细胞损伤减少,电镜可见灵芝多糖(50mg.L-1,温育24h)减轻细胞器如线粒体氧化损伤,减少细胞凋亡。细胞流式检测表明:对照组、给药组、损伤组总凋亡百分率分别2.24%±0.43%、24%±6.4%(P<0.01)、82.1%±7.9%。结论灵芝多糖肽(GLPP)对ECV304细胞氧化损伤具有保护作用。

Ganoderma lucidum polysaccharides peptide (GLPP) protects ECV304 cells from oxidative injury

Aim To study the protective effects of Ganoderma lucidum polysaccharides peptide(GLPP)on ECV304 from oxidative injury.Methods Cultured ECV304 were injured by oxygen free radicals derived from tBOOH.Various concentrations of GLPP(12.5,25,50,100 mg·L-1)were added into culture medium.The survival rate of cells was measured by MTT assay.The morphological change of cells and injury of mitochondria were examined under the light and electron microscopes.The percentage of apoptosis of ECV304,labeled with AnnexinV/PI,was measured by flow cytometry.Results GLPP(12.5,25,50,100 mg·L-1)could reduce oxidative injury induced by tBOOH in ECV304 cells.The survival rate of cells treated with GLPP increased.The light microscopic examination showed that the injured cells decreased in GLPP-treated groups.Under the electron microscope it was found that GLPP(50 mg·L-1,incubated for 24 h)could protect the organelle such as mitochondria from oxidative injury and cells from apoptosis by tBOOH.The result of flow cytometry showed that the total percentage of apoptosis in control,GLPP and injury treated group was 2.24%±0.43%,24±6.4%(P<0.01),and 82.1%±7.9% respectively.Conclusions GLPP had protective effects on ECV304 from oxidative injury.