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| Ser~(202)/Thr~(205)位点磷酸化tau在神经元中的定位 | |
| 引用本文： | 孙爱民,王海萍,沈玉君,沈玉先,方圣云.Ser~(202)/Thr~(205)位点磷酸化tau在神经元中的定位[J].中国药理学通报,2011,27(1):41-45. |
| 作者姓名： | 孙爱民 王海萍 沈玉君 沈玉先 方圣云 |
| 作者单位： | 1. 安徽医科大学基础医学院,安徽,合肥,230032;安徽医科大学生物药物研究所,安徽,合肥,230032 2. 安徽医科大学基础医学院,安徽,合肥,230032 3. 安徽医科大学生物药物研究所,安徽,合肥,230032 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的观察Ser202/Thr205位点的过度磷酸化tau在神经元中的定位和聚集情况。方法取孕18 d SD胎鼠皮层神经元进行原代培养,于培养第9天加入冈田酸(okadaic acid,OA)处理12 h以诱导tau蛋白磷酸化;同时用OA诱导SH-SY5Y细胞;观察tau磷酸化后神经元形态的变化;用AT-8抗体免疫荧光染色观察Ser202/Thr205位点磷酸化tau的定位和聚集;同时应用AT-8抗体和Tau-5抗体检测tau蛋白Ser202/Thr205位点的磷酸化水平变化情况。结果经过Anti-NeuN和Anti-GFAP免疫染色鉴定,孕18 d胎鼠皮层神经元选择性原代培养成功。OA处理12 h以后,Ser202/Thr205位点磷酸化tau的水平明显增加,且出现高分子量的寡聚体;免疫荧光染色发现,Ser202/Thr205位点磷酸化的tau在原代培养神经元的胞质聚集;而OA处理的SH-SY5Y细胞,Ser202/Thr205位点磷酸化的tau却定位于细胞核。同时发现OA处理的原代神经元突起明显减少或消失。结论 OA能够诱导原代培养的胎鼠神经元tau蛋白在Ser202/Thr205位点过度磷酸化,且该磷酸化tau在不同类型或来源的神经元中的定位不同。 |
| 关 键 词： | 冈田酸 胎鼠皮层神经元 tau 过度磷酸化 阿尔采末病 磷酸化位点 |
Differential induction of tau phosphorylation by OA treatment in primarily cultured neurons and SH-SY5Y cells |
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| SUN Ai-min,WANG Hai-ping,SHEN Yu-jun,SHEN Yu-xian,FANG Sheng-yun.Differential induction of tau phosphorylation by OA treatment in primarily cultured neurons and SH-SY5Y cells[J].Chinese Pharmacological Bulletin,2011,27(1):41-45. | |
| Authors: | SUN Ai-min WANG Hai-ping SHEN Yu-jun SHEN Yu-xian FANG Sheng-yun |
| Institution: | SUN Ai-min1,2,WANG Hai-ping1,SHEN Yu-jun2,SHEN Yu-xian1,FANG Sheng-yun1(1.School of Basic Medical Sciences,2.Institute of Biopharmaceutical Research,Anhui Medical University,Hefei 230032,China) |
| Abstract: | Aim To investigate the characteristics of tau phosphorylation induced by OA treatment in primarily cultured embryonic neurons and SH-SY5Y cells.Methods Eighteen-day Sprague Dawley embryos were sacrificed to perform primary cultivation of cortical neurons.After treatment with OA for 12 h on day 9,the neurons were subject to immunocytochemistry and Western blot using Ser202/Thr205 phosphorylation-dependent antibody AT-8.Tau-5 antibody was also used as a counterpart to recongnize the total tau protein.Results ... |
| Keywords: | tau |
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