| 五味子乙素对染矽尘大鼠肺组织一氧化氮水平和诱导型一氧化氮合酶mRNA表达动态变化的影响 |
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| 引用本文： | 樊林花,刘田福,郭民,刘茂林.五味子乙素对染矽尘大鼠肺组织一氧化氮水平和诱导型一氧化氮合酶mRNA表达动态变化的影响[J].中国药理学通报,2011,27(2):225-228. |
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| 作者姓名： | 樊林花 刘田福 郭民 刘茂林 |
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| 作者单位： | 山西医科大学实验动物中心,山西,太原,030001 |
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| 基金项目： | 山西省实验动物专项基金 |
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| 摘 要： | 目的观察五味子乙素(Sch-B)对染矽尘大鼠肺组织一氧化氮(NO)水平和诱导型一氧化氮合酶(iNOS)mRNA动态变化的影响。方法将96只大鼠随机分为对照组、染矽尘组、Sch-B组,每组32只,气管暴露法建立大鼠矽肺模型,造模后d 1开始灌胃给予Sch-B治疗,药物治疗3 d、7 d、14 d和28 d后,HE染色检测肺组织病理改变;硝酸还原酶法测肺组织NO含量;RT-PCR检测肺组织iNOS mRNA的表达。结果 HE染色显示Sch-B组大鼠肺损伤较染矽尘组明显减轻。NO含量和iNOS mRNA表达在染尘后各个时间点均较相应的对照组明显升高(P<0.01),其中NO含量在d 7时达到高峰后开始下降,iNOS mRNA的峰值出现在d 14。五味子组与染矽尘组相比,各时间点NO含量均明显降低(P<0.05或P<0.01),而iNOS mRNA表达仅在d 3和d 7时降低明显(P<0.05)。结论染矽尘大鼠肺组织存在着NO含量和iNOS mRNA的动态变化。Sch-B能减轻染矽尘大鼠肺组织的纤维化程度,其机制可能与染尘初期Sch-B降低iNOS mRNA转录水平,抑制NO炎症介质的合成与释放有关。
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| 关 键 词： | 五味子乙素 二氧化硅 肺纤维化 一氧化氮 诱导型一氧化氮合酶 大鼠 |
Effect of schisandrin B on dynamic change of content of nitric oxide and mRNA expression of inducible nitric oxide synthase in rat lungs exposed to silica |
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| FAN Lin-hua,LIU Tian-fu,GUO Min,LIU Mao-lin.Effect of schisandrin B on dynamic change of content of nitric oxide and mRNA expression of inducible nitric oxide synthase in rat lungs exposed to silica[J].Chinese Pharmacological Bulletin,2011,27(2):225-228. |
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| Authors: | FAN Lin-hua LIU Tian-fu GUO Min LIU Mao-lin |
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| Institution: | FAN Lin-hua,LIU Tian-fu,GUO Min,LIU Mao-lin(Laboratory Animal Center,Shanxi Medical University,Taiyuan 030001,China) |
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| Abstract: | Aim To investigate the effect of schisandrin B on content of nitric oxide(NO)and expression of inducible nitric oxide synthase(iNOS)in rat lungs exposed to silica.Methods Ninty six Wistar rats were randomly divided into control group,silica group and Sch-B treated group with 32 rats in each group.Silicotic animal models were established by direct tracheal instillation of silica into rat lungs surgically.From the first day after model establishment,rats in Sch-B treated group were orally given Sch-B.At 3,7,1... |
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| Keywords: | schisandrin B silica pulmonary fibrosis nitric oxide inducible nitric oxide synthase rats |
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